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Linking vitamin B1 with cancer cell metabolism

Cancer & Metabolism volume 1, Article number: 16 (2013) Cite this article

Abstract

The resurgence of interest in cancer metabolism has linked alterations in the regulation and exploitation of metabolic pathways with an anabolic phenotype that increases biomass production for the replication of new daughter cells. To support the increase in the metabolic rate of cancer cells, a coordinated increase in the supply of nutrients, such as glucose and micronutrients functioning as enzyme cofactors is required. The majority of co-enzymes are water-soluble vitamins such as niacin, folic acid, pantothenic acid, pyridoxine, biotin, riboflavin and thiamine (Vitamin B1). Continuous dietary intake of these micronutrients is essential for maintaining normal health. How cancer cells adaptively regulate cellular homeostasis of cofactors and how they can regulate expression and function of metabolic enzymes in cancer is underappreciated. Exploitation of cofactor-dependent metabolic pathways with the advent of anti-folates highlights the potential vulnerabilities and importance of vitamins in cancer biology. Vitamin supplementation products are easily accessible and patients often perceive them as safe and beneficial without full knowledge of their effects. Thus, understanding the significance of enzyme cofactors in cancer cell metabolism will provide for important dietary strategies and new molecular targets to reduce disease progression. Recent studies have demonstrated the significance of thiamine-dependent enzymes in cancer cell metabolism. Therefore, this review discusses the current knowledge in the alterations in thiamine availability, homeostasis, and exploitation of thiamine-dependent pathways by cancer cells.

Review

Vitamin B1

Thiamine is classified as an essential water-soluble vitamin requiring continuous dietary intake to support carbohydrate metabolism. Thiamine is critical for the activity of four key enzymes in cellular metabolism, pyruvate dehydrogenase (PDH) and alpha-ketoglutarate dehydrogenase (α- KGDH) in the tricarboxylic acid (TCA) cycle, transketolase (TKT) within the pentose phosphate pathway (PPP), and branched chain alpha-keto acid dehydrogenase complex (BCKDC) involved in amino acid catabolism. Structurally, thiamine is composed of a thiazole and pyrimidine ring joined together by a methylene bridge (Figure 1). Although thiamine is not the co-enzyme but is converted to the active diphosphate, thiamine pyrophosphate (TPP) form intracellularly, circulating plasma levels of thiamine in healthy individuals range between 10 and 20 nM [1]. The recommended daily intake (RDI) of thiamine for adult men and women is approximately 1–1.5 mg/day [2]. Thiamine is found naturally in many foods including breads, fish, meat, eggs, legumes and milk, as well as being used in fortification of many processed foods (Table 1). In addition, many over-the-counter vitamin supplements contain a significantly large quantity of thiamine representing 100 to 6,600% of the RDI (Table 1).

Table 1 Amount and percent daily value (DV) of thiamine found in food and supplements
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Figure 1
figure 1

Chemical structures of thiamine (vitamin B1; thiamin) and the active co-enzyme thiamine pyrophosphate (thiamine diphosphate; TPP).

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Vitamin B1 homeostasis

The quaternary nitrogen and overall hydrophilicity of thiamine necessitates a carrier-mediated process for absorption and cellular uptake (Figure 2). Two transporters belonging to the SLC19A family, THTR1 (SLC19A2) and THTR2 (SLC19A3) primarily facilitate the transport of thiamine. The other member of the SLC19A family, RFC1 (SLC19A1) facilitates intracellular uptake of reduced folate [3]. Although all three transporters share a high degree of amino acid sequence similarity, RFC1 does not transport thiamine and THTR1/2 has not been shown to transport reduced folate or other organic cations [4, 5]. SLC19A2 and SLC19A3 transport thiamine with Km values of 2.5 μM and 27 nM, respectively [6, 7]. Intestinal absorption of thiamine has also been described to occur by passive diffusion mechanisms at high concentrations and also via members of the organic cation transporter family [8–10]. Recently, a high affinity carrier-mediated transport mechanism for TPP has been characterized in intestinal cells but no transporter has been identified [11].

Figure 2
figure 2

Intracellular thiamine homeostasis is initially achieved through the uptake of thiamine (T) by the thiamine transporters THTR1 and THTR2. Once inside the cell, thiamine is converted to the active co-enzyme, thiamine pyrophosphate (TPP) by thiamine pyrophosphokinase-1 (TPK1). Thiamine can than function as a cofactor for the cytoplasmic TKT. Transport of TPP by the thiamine pyrophosphate carrier (TPC) across the mitochondrial membrane supplies cofactor for activity of pyruvate dehydrogenase (PDH) and alpha-ketoglutarate dehydrogenase (α-KGDH). Intracellular TPP can also be converted to thiamine monophosphate (TMP) by thiamine pyrophosphatase (TPPase) and subsequently recycled back to thiamine by thiamine monophosphatase (TMPase). Both TMP and TPP can be effluxed out of the cell through the reduced folate carrier (RFC1).

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Upon transport into the cell, thiamine is converted to the active co-enzyme thiamine pyrophosphate (diphosphate) by thiamine pyrophosphokinase-1 (TPK1) (Figure 1) [12]. Phosphorylation of thiamine by TPK1 has been shown to be a significant driving force for thiamine uptake along with binding to apo-enzymes [13]. Human TPK1 exists as a homodimer and is expressed ubiquitously with high levels in the kidney, small intestine, and testis [12, 14]. In addition to TPP, three other phosphorylated forms have been observed intracellularly in humans, thiamine monphosphate (TMP), thiamine triphosphate (TTP), and adenosine thiamine triphosphate (AThTP) [1]. Although the physiological functions of TMP, TTP, and AThTP have not been ascertained, TPP is the only known thiamine phosphorylate functioning as an enzyme cofactor. Dephosphorylation of intracellular TPP by thiamine pyrophosphatase to TMP can be subsequently recycled back to free thiamine via thiamine monophosphatase [15, 16]. SLC19A1 has been shown to efflux the mono and diphosphate ester of thiamine [17]. It is unclear if the dephosphorylation and efflux of thiamine phosphorylates is to regulate intracellular thiamine levels to control cofactor and non-cofactor functions of thiamine phosphorylates. Transport of TPP across the mitochondrial membrane to support PDH and α-KGDH activity is facilitated by the thiamine pyrophosphate carrier (TPC), which is encoded by the SLC25A19 gene [18]. Previously identified as a deoxynucleotide carrier or DNC, homology comparisons to yeast TPP transporter and transport assays have clearly identified TPC as a TPP transporter [18, 19].

Thiamine homeostasis genes and cancer

A difference in the expression of thiamine homeostasis genes in cancer has been extensively demonstrated for the thiamine transporter SLC19A3. Using a cDNA array, Liu et al. demonstrated a decrease in SLC19A3 expression in breast cancer compared to corresponding normal tissue [20]. Down-regulation of SLC19A3 was also found in gastric and colon cancer [21, 22]. The decrease in expression appears to involve epigenetic repression through hypermethylation and histone deacetylation of the SLC19A3 promoter [21, 22]. An elevation of SLC19A3 methylation was detected in the plasma of early- and advanced-stage breast cancer patients as well as gastric cancer [23]. Recently, our group discovered a significant increase in the gene expression of TPK1, SLC19A2, and SLC25A19 in breast cancer tissue samples compared to normal breast tissue [24]. A slight decrease in SLC19A3 gene expression was also found in concordance with previous findings. Upregulation of TPK-1, SLC19A2, SLC25A19 and downregulation of SLC19A3 was also verified in several breast cancer cell lines compared to human mammary epithelial cells (hMECs). Although SLC19A3 expression was repressed, transport assays demonstrated an increase uptake of thiamine in all breast cancer cell lines tested compared to hMECs. Using HPLC to quantify thiamine and thiamine phosphorylates, the overall thiamine status (total of all thiamine forms assayed) was significantly greater in two of the four cancer cell lines evaluated compared to hMECs. Interestingly, all four breast cancer cell lines exhibited a high level of intracellular free thiamine compared to TPP. This may suggest an important non-cofactor role of thiamine in cancer cells or a mechanism to retain the intracellular thiamine supply to support binding to newly synthesized apo-enzyme in progenitor or newly formed daughter cells.

Why tumor cells repress the expression of THTR2 is still unclear. Decreasing expression of one thiamine transporter may not impact overall thiamine status, since transport may still occur via THTR1. This may indicate additional functionality of THTR2 beyond facilitating the intracellular transport of thiamine. When exogenously re-expressing SLC19A3 in gastric cancer cells, a marked decrease in growth rate was found [21]. Liu et al. demonstrated that THTR2 transfection in breast cancer cells increased sensitivity to ionizing radiation and cytotoxicity to doxorubicin [21]. However, it is unclear if the growth-suppressive properties observed in these two studies are mediated through an increase in cellular thiamine concentrations from enhanced transport capacity or due to a novel pro-apoptotic function of THTR2. To this end, Liu et al. established the changes in gene expression when overexpressing THTR2 and when grown in thiamine-deficient media [25]. Several genes involved in oncogenesis were upregulated with THTR2 overexpression that was reduced in the absence of exogenous thiamine [25]. The increase in oncogenic genes with THTR2 re-expression would appear to contradict a role for THTR2 as a tumor suppressor but highlights a potential role for thiamine and THTR2 expression in tumorigenesis.

Though the expression of SLC19A3 appears to be repressed in a number of cancers, Sweet et al. demonstrated that hypoxic exposure resulted in upregulation or re-establishment of SLC19A3 expression in breast cancer cells [26]. A corresponding 2-fold increase in thiamine transport was also observed, suggestive of an increased requirement for thiamine during hypoxic stress [26]. The presence of hypoxic microenvironments in tumors results in an increase in glycolytic activity, primarily controlled by the hypoxia-inducible factor-1 alpha (HIF-1α) transcription factor. Knockdown of HIF-1α attenuated the upregulation, suggesting that SLC19A3 may be an associated gene involved in the adaptive metabolic shift during hypoxic stress [26]. Interestingly, thiamine was shown to reduce hypoxia-mediated apoptosis of rat cardiomyocytes [27]. Thus, increasing transport activity and intracellular thiamine supply may be part of a pro-survival response during hypoxic stress. In this capacity, it is unclear whether thiamine is functioning as a cofactor or non-cofactor. Evidence suggests that the mitochondrial injury associated with hypoxia can lead to an imbalance of reactive oxygen species (ROS) in cancer cells [28–30]. Unchecked, the excess ROS can lead to cellular apoptosis and necrosis and has been exploited as a chemotherapeutic approach [31, 32]. Thiamine has direct antioxidant properties as well as being essential for glutathione production [33–35]. Therefore, clarification of thiamine’s role in pro-survival responses to hypoxic stress would be of great significance, given the association of hypoxia with poor patient prognosis.

Thiamine and cancer

It has been hypothesized that a Western diet, characterized in part by excess thiamine supplementation, may be a factor for increased cancer incidence compared to other countries [36]. Thiamine is commonly supplemented in processed foods and readily consumed in over-the-counter vitamin and nutritional supplements in Western countries with generally high cancer incidences. In contrast, Asian and African countries principally consume food that is high in thiaminase, a natural thiamine-degrading enzyme, which may reduce thiamine exposure [36]. Although no direct studies have evaluated this hypothesis, several have attempted to correlate the intake of thiamine and other nutritional components with the risk of cancer. However, like so many other nutritional correlations with cancer incidence, the dietary intake of thiamine and cancer risk has provided conflicting results. Using nutritional questionnaires and a calculated average daily intake level, patients with prostate cancer consume less thiamine than those without cancer suggesting a negative association with cancer risk [37]. A 2008 study examined the relationship between the intake of B vitamins and incidence of breast, endometrial, ovarian, colorectal, and lung cancer in women [38]. No correlation was found between intake of the B vitamins, including thiamine, riboflavin, niacin, and folate, and the incidence of cancer. Interestingly, reduced thiamine intake increased the number of aberrant crypt foci in the colons of rats fed a sucrose-based diet [39]. Patients with severe malnutrition have exhibited Baker’s cyst, osteosarcoma, and submandibular gland cysts that were cured without recurrence after thiamine administration, suggesting a role of thiamine deficiency in tumor development [40].

A limited number of studies and case reports have determined the overall thiamine status in cancer patients. Clinically, thiamine status is quantified biochemically using a TKT assay of whole blood samples [41]. This assay involves measuring the increase in the activity of the thiamine-dependent enzyme TKT after added TPP. If deficient in thiamine, exogenous TPP will stimulate TKT activity, termed the TPP effect. Basu et al. demonstrated that patients with advanced cancer exhibit a greater TPP stimulating effect, suggestive of a reduced thiamine status [42, 43]. Similarly, increased TPP effect was characterized in patients with B-chronic lymphocytic leukemia, Burkett’s lymphoma, and acute myelomonocytic leukemia [44, 45]. Using an HPLC assay to directly quantify TPP levels in whole blood, Tsao et al. demonstrated a significant decrease of TPP in patients with advanced stages of non-small cell lung cancer [46]. Although the reason for a decrease in thiamine status in the blood is unclear, one study noted that cancer patients had a higher level of thiamine urinary excretion [42]. The authors suggested that the decrease in thiamine status might not be due to reduced dietary intake of thiamine, but an inability to activate thiamine to TPP [42]. However, thiamine status is primarily assayed biochemically in whole blood and limited studies have quantified thiamine/TPP directly in cancer tissue. The reductions in peripheral thiamine/TPP may be a consequence of extensive accumulation and/or utilization by cancer cells. Trebukhina et al. demonstrated that tumor growth resulted in a depletion of tissue vitamin stores and an increase in the TPP-stimulating effect in blood [47]. During tumor growth, cancer cells maintained a constant level of TPP while host liver tissue exhibited a perpetual decline [48]. In post-surgical or autopsy tissues, a 2.5-fold increase in thiamine levels was found in colon adenocarcinomas relative to un-invaded control tissue [49]. Overall these studies strongly suggest a preferential accumulation of thiamine into cancer cells that may be responsible for the alteration in peripheral thiamine status during malignancy.

Aside from the disease itself, chemotherapeutic drugs such as 5-fluorouracil (5-FU) and ifosfamide have been associated with inducing a thiamine-deficient state in patients [50, 51]. In most cases, patients exhibit neurological impairment similar to the sequelae observed in thiamine deficiency conditions such as Wernicke’s encephalopathy [51–54]. How these drugs that are structurally unrelated to thiamine are capable of inducing a deficiency is unknown and may involve distinct mechanisms. No change in thiamine or TPP levels was found in patients receiving ifosfamide treatment, suggesting that ifosfamide or a metabolite may inhibit a thiamine-dependent pathway [51, 55]. Accumulation of thiamine was found to be increased in cancer cells and rat hepatocytes when treated with 5-FU and doxifluridine [56]. The enhancement in thiamine uptake by 5-FU in cancer cells has been associated with an increase in intracellular TPP while free thiamine remained constant [56]. Treatment of rats for three consecutive days with 5-FU was found to result in an increase in the TPP-stimulating effect on whole blood TKT, and a decrease in liver thiamine stores [57]. Thus, 5-FU appears to decrease peripheral thiamine levels by increasing cellular accumulation and conversion to TPP. Although the mechanism for either drug still needs continued research, high-dose thiamine is capable of reversing the symptoms of thiamine deficiency [50, 51, 53, 54].

Self-supplementation vitamin preparations containing levels of thiamine greater than the RDI are readily accessible and considered to be safe and harmless for patients (Table 1). Although the use of thiamine to treat deficiency-related symptoms attributed to the disease or therapy is warranted, this is currently done with limited comprehension of the role thiamine may contribute towards malignant progression. In light of our knowledge regarding alterations of thiamine homeostasis in cancer, the impact of thiamine supplementation on cancer growth has received minimal research attention. In 2001, Comin-Anduix et al. evaluated the effect of increasing thiamine supplementation in multiples of the RDI on an Ehrlich ascites tumor-mouse model [58]. Their findings indicated a statistically significant stimulatory effect of thiamine supplementation on tumor growth compared to non-supplemented controls. Moderate doses of 12.5 to 37.5 times the RDI had the greatest stimulatory effect, peaking at approximately 250% greater tumor cell proliferation with 25 times the RDI. Interestingly, at values above 75 times the RDI, no change was found in tumor cell proliferation, and a slight decrease was found at 2,500 times the RDI. This observation suggests that there is a specific range in which thiamine supports proliferation. A recent study explored the relationship between a high-fat diet and thiamine levels on the tumor latency in the Tg(MMTVneu) spontaneous breast cancer-tumor mouse model [59]. In this study a normal-fat (NF) diet contained 10% of the calories from fat while the high-fat diet contained 60%. Low thiamine (LT) levels were defined as 2 mg of thiamine per 4,057 kcal and normal thiamine (NT) levels as 6 mg per 4,057 kcal. Tumor latency was significantly longer (295 days) in animals given a NF/LT diet compared with animals on NF/NT (225 days). Interestingly,the delay in tumor latency from LT was abolished when given a high-fat diet. This demonstrates an important interplay of dietary constituents on tumor progression that needs further characterization. Although more research is needed to confirm and evaluate the role of thiamine on disease progression, these studies have significant clinical implications. First, patients requiring thiamine to treat either chemotherapy or disease-associated deficiency should receive high-dose thiamine to avoid enhancing tumor growth. Second, self-supplementation of thiamine by cancer patients should be avoided as the low-to-moderate levels of thiamine may contribute to disease exacerbation.

The importance of thiamine in cancer cell proliferation is highlighted by studies using the thiamine-degrading enzyme thiaminase. Liu et al. demonstrated that the addition of thiaminase into cell culture media containing thiamine had a significant growth inhibitory effect on breast cancer cells [60]. Thiaminase reduced ATP levels in cancer cells, demonstrating thiamine’s key role in support of cancer cell bioenergetics. Moreover, a pegylated version of thiaminase was capable of delaying tumor growth and prolonging survival in an RS4 leukemia xenograft model [61]. Thiamine’s key role in cancer cell metabolism and survival is further demonstrated by studies using the thiamine analog oxythiamine, which functions as an anti-coenzyme and is capable of reducing in vivo and in vitro tumor cell growth [62–64]. Inhibition of TKT by oxythiamine reduces DNA and RNA synthesis through reductions in ribose 5-phosphate (R5P) synthesis, the pentose carbon backbone of all nucleotides. Oxythiamine also has been shown to induce apoptosis in rat PC-12 cells via mitochondria-dependent caspase 3-mediated signaling pathways [65]. The effect on nucleotide synthesis is highlighted by the prominent G1 cell cycle arrest induced by oxythiamine in Ehrlich’s tumor cells [63]. Yang et al. demonstrated that oxythiamine decreases cell migration and invasion in vitro, as well as reduced lung metastases in mice with Lewis lung carcinoma (LLC) [66]. Interestingly, oxythiamine did not reduce the proliferation of LLC cells at concentrations that reduced migration and invasion. The effect of oxythiamine was attributed to a dose-dependent reduction in MMP-2 and MMP-9 activity and expression. This finding suggests that thiamine-dependent pathways have other repercussions on cancer progression in addition to effects on cellular proliferation.

Thiamine-dependent enzymes in cancer

Transketolase

The PPP and in particular the thiamine-dependent enzyme TKT is essential for cancer cells to synthesize large amounts of nucleic acids needed for rapid cellular growth (Figure 3). In normal cells, glucose 6-phosphate enters the PPP and is converted to the nucleotide pentose sugar R5P through the non-thiamine-dependent oxidative branch. If not utilized for de novo nucleotide synthesis, R5P continues into the non-oxidative branch of the PPP where TKT ultimately converts R5P into fructose 6-phosphate (F6P) and glyceraldehyde 3-phosphate (G3P), which re-enters the Embden-Meyerhoff pathway. In contrast, a shift to increased reliance on the non-oxidative PPP for R5P production is found in cancer cells. Boros et al. demonstrated that 98% of the ribose molecules in H441 lung cancer cells were derived through the non-oxidative pathway [67]. Similarly, 85% of the ribose RNA in pancreatic adenocarcinoma cells was from the non-oxidative pathway [62].

Figure 3
figure 3

Under normal cell metabolism G6P entering the oxidative pentose phosphate pathway (PPP) is converted to ribose 5-phosphate (R5P) and xylulose 5-phsophate (X5P). Both can be further metabolized through the non-oxidative pathway to ultimately form fructose 6-phosphate (F6P) and glyceraldehyde 3-phosphate (G3P) that re-enters the glycolytic pathway to continue catabolism for ATP production. In cancer, reduced activity of M2-PK leads to an excess of F6P and G3P that can be shunted back into the non-oxidative pathway for anabolism. Mediated through transaldolase (TA) and the TPP-dependent enzyme TKT, F6P and G3P are converted to R5P for biosynthesis of nucleotides.

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One of the key regulators of glycolysis in cancer cells is the M2 isoform of pyruvate kinase (M2-PK), which catalyzes the conversion of phosphoenolpyruvate to pyruvate. M2-PK is highly expressed in rapidly proliferating cells and is the predominant isoform in cancer [68]. Depending on the metabolic needs, M2-PK oscillates between the active tetramer and inactive dimeric form [69]. The dimer, also known as tumor M2-PK, is the predominant form of PK found in cancer cells and is a potential biomarker for cancer detection [70–73]. The reduced PK activity induces a build-up of phospho-metabolites such as F6P and G3P that are utilized by TKT to produce R5P [74]. Recently, metabolic profiling of gliomas was consistent with an anabolic signature exhibiting reduced M2-PK activity, a build-up of glycolytic intermediates, and high R5P production [75]. Resistance of chronic myeloid leukemia cells to imatinib is associated with an increase in M2-PK expression and an increase in glucose flux into RNA through the non-oxidative PPP [76, 77]. Thus, the alteration in glycolysis regulation redirects glucose carbon into thiamine-dependent anabolic pathways that support rapid proliferation and cell survival.

Although the TKT reaction is generally considered to be the result of a single TKT gene, there are two additional TKT isoforms found in the human genome, termed TKT-like 1 (TKTL1) and TKT-like 2 (TKTL2). The increase in substrate flux through the non-oxidative PPP may be supported by an overexpression of TKT isoforms. Immunohistochemical staining of malignant tissues, including breast, lung, colonic, urothelial, ovarian, endometrial, gastric and laryngeal tissues, have all exhibited an overexpression of TKTL1 compared to normal tissues [78–86]. High expression of TKTL1 is correlated with tumor progression and poor patient prognosis [78, 79, 85, 87]. TKTL1 silencing has been shown to suppress growth and proliferation within various tumor cell lines and xenograft models [88–93]. Ectopically overexpressing TKTL1 in head and neck squamous cell carcinoma cells were found to increase cellular proliferation over vector control [94]. Cells overexpressing TKTL1 consumed more glucose, produce greater amounts of G3P, F6P, pyruvate, lactate, and R5P, indicating that TKTL1 promotes an aerobic glycolysis phenotype. Overexpression of TKTL1 has also been associated with activation of the pro-oncogenic HIF-1α under normoxic conditions [94]. The PPP and TKTL1 activity may be important in pro-survival responses by limiting excess production of ROS when challenged with oxidative stresses, such as hypoxia. The glycolytic flux through the oxidative PPP generates nicotinamide adenine dinucleotide phosphate (NADPH), which is essential in regenerating glutathione and for biosynthetic reactions. Increased oxidative stress in cancer cells was demonstrated to suppress M2-PK activity and enhance flux through the oxidative PPP with a concomitant decrease in reduced glutathione levels [95]. Interestingly, knockdown of TKTL1 expression was found to reduce cellular NADPH and glutathione levels with a reciprocal increase in ROS-mediated apoptosis [91]. When exposed to hypoxic stress, reducing TKTL1 expression has resulted in an increase in ROS generation and cell death to glioma cells [96]. It is unclear how TKTL1 activity in the non-oxidative PPP is related to NADPH production generated within the oxidative pathway. TKTL1 activity may contribute to NADPH formation by maintaining continuous flux of glucose carbons through the PPP to avoid intermediate feedback on enzymatic activity.

Although the importance of TKTL1 in cancer proliferation, survival, and metabolism is well established, several reports have suggested that TKTL1 is incapable of enzymatically functioning as a TKT. Homology comparisons of TKTL1 with TKT have noted a deletion of 38 amino acids within the cofactor and catalytic domain, suggesting that TKTL1 is incapable of binding to TPP and carrying out the TKT reaction [97, 98]. Schneider et al. engineered a 38 amino acid deletion pseudo-TKTL1 (TKTΔ38) mutant from TKT as a model of TKTL1 to elucidate this issue [99]. No conventional TKT activity of the TKTΔ38 mutant was detectable using a coupled spectrophotometric assay for the conversion of known physiological substrates. Moreover, circular dichroism and proton nuclear magnetic resonance (1H-NMR) spectroscopy indicated that the TKTΔ38 mutant had no associated TPP. Using the same TKTΔ38 mutant system, Meshalkina et al. confirmed the lack of TKT activity in solution and were unable to detect TPP after acid or heat denaturation extraction methods [100]. However, the lack of activity of purified TKTΔ38 in solution contradicts reports describing the TKT activity of TKTL1 when exogenously overexpressed or repressed in cancer cells [76, 91, 93, 94]. Since TKT functions as a homodimer, it is unclear if the TKTΔ38 mutation alters dimer formation. Even if TKTL1 is enzymatically inactive as a homodimer, the expression of TKTL1 in mammalian systems may influence overall TKT activity through heterodimer formation with other TKT isoforms. The lack of TPP binding to the TKTΔ38 mutant does not preclude other thiamine derivatives from binding. The diphosphate group added to thiamine does not participate in the catalytic activity and functions primarily to anchor the cofactor into the apo-enzyme. Other thiamine derivatives with unknown function have been found intracellularly; these may be able to bind within the condensed catalytic site of TKTL1 [1]. Thus, further work is needed to fully understand the biochemistry of TKTL1 in mammalian systems.

How thiamine supplementation impacts carbon flux through the non-oxidative pathway and modulates TKT activity in cancer cells is unknown. Increases in TKT activity may be attributed to an upregulation of enzyme expression. Additionally, thiamine supplementation may also stimulate TKT activity by maintaining a high holo-enzyme fraction by binding to apo-enzymes in progenitor and/or newly generated daughter cells. Recent work describing the benefit of thiamine supplementation in reducing hyperglycemia-induced vascular damage in diabetes may provide some insight. Similar to cancer, hyperglycemia can result in a build-up of phosphometabolites in non-insulin-dependent tissues such as the vascular endothelia. This diverts glucose metabolites into the polyol, hexosamine, advanced glycation, and the diacylglycerol pathways, which are associated with inducing hyperglycemic vascular damage [101]. High-dose thiamine, or the thiamine derivative benfotiamine, stimulate TKT activity and decrease production of toxic metabolites [102–104]. The exposure of high-dose thiamine to human umbilical vein endothelial cells and bovine retinal pericytes cultured in high glucose has been found to result in an increase in TKT mRNA expression and TKT activity [105]. Conversely, thiamine deficiency was found to decrease TKT mRNA levels and reduce TKT activity in neuroblastoma cells [106]. Thus, thiamine appears to have both a regulatory and stimulatory effect on TKT activity.

Pyruvate dehydrogenase

The conversion of pyruvate to acetyl-CoA takes place through a series of reactions mediated by the thiamine-dependent enzyme PDH. Located within the mitochondrial matrix, PDH is a multi-component enzyme complex consisting of three subunits (E1, E2, and E3). As a result of its location at the junction between glycolysis and the TCA cycle, PDH activity functions as a critical gatekeeper for the continued metabolism of glucose (Figure 4). The activity of PDH is tightly regulated through phosphorylation by PDH kinase (PDK) [107]. PDK is a family of four isoenzymes (PDK1, 2, 3 and 4) that function to inhibit PDH activity through ATP-dependent phosphorylation [108–110].

Figure 4
figure 4

The pyruvate dehydrogenase (PDH) complex converts pyruvate into acetyl-CoA when bound to the co-enzyme, thiamine pyrophosphate (TPP). In cancer, phosphorylation of PDH by pyruvate dehydrogenase kinase isoforms 1 to 4 (PDK1 to 4) inactivates PDH, leading to a reduction, in pyruvate conversion to lactate by lactate dehydrogenase A (LDHA). Inhibition of PDK activity by dichloroacetate (DCA) reduces phosphorylation and induces apoptosis. TPP binding to PDH has also been suggested to inhibit PDK phosphorylation and may explain why high dose thiamine has anti-proliferative effects in a tumor xenograft model. In the oxidative direction, pyruvate is converted to acetyl-CoA by PDH and is continually catabolized to α- ketoglutarate. The thiamine-dependent enzyme alpha-ketoglutarate dehydrogenase (α- KGDH) converts α- ketoglutarate to succinyl-CoA. Cancer cells exploit glutaminolysis to resupply the tricarboxylic acid (TCA) cycle with carbon as α- ketoglutarate. Continuation of the TCA cycle results in anabolic activity to provide precursors for nucleotides, amino acids, and lipids for biomass generation.

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In normal cells PDH is active, allowing cells to maintain oxidative metabolism to produce ATP and other components necessary for cell survival and proliferation. However, PDH activity is suppressed in cancer due to downregulation and overexpression of PDK isoforms [111–114]. This truncation of glucose metabolism, highlighted by preferential conversion of pyruvate to lactate, provides cancer cells with a metabolic advantage to maintain rapid proliferation [115]. Thus, an increase in thiamine availability to cancer cells would not be expected to further increase carbon flux through PDH. However, PDH expression is elevated with a reciprocal decrease in PDK isoform expression in tumor-associated stromal tissue, such as fibroblasts and vascular endothelial cells [114, 116]. The increased PDH activity in surrounding tissues has been proposed to assist in the detoxification of extracellular lactate produced by cancer cells [116]. This metabolic symbiosis between cancer cells and stromal tissue may be enhanced through increasing thiamine availability, promoting PDH activity in normal tissue surrounding tumors.

Interestingly, restoration of PDH activity in cancer cells has been shown to promote apoptosis and is actively being assessed as a potential therapeutic strategy. One such compound, DCA, has shown considerable promise in multiple cancer types to inhibit PDK-mediated phosphorylation of PDH [117, 118]. The re-establishment of glucose oxidation in cancer cells through restored PDH activity results in an increase in mitochondrial ROS and cytochrome C release, ultimately leading to apoptosis [119, 120]. Regulation of PDK activity is mediated through the accumulation of metabolic products such as ATP, nicotinamide adenine dinucleotide (NADH) and acetyl-CoA, which stimulate activity, while pyruvate and ADP inhibit when in excess [121]. Another regulator of PDH phosphorylation is the thiamine cofactor TPP, which, when bound to PDH, reduces the rate and extent of PDK-mediated phosphorylation [110]. Thus increasing concentrations of TPP through thiamine supplementation may be pro-apoptotic through restoration of PDH activity in cancer cells. This may explain why a reduction in tumor growth was observed with high-dose thiamine supplementation [58]. The potential of high-dose vitamin B1 to reduce cancer cell growth would be of particular significance and warrants further study.

Alpha-ketoglutarate dehydrogenase

In addition to glucose, cancer cells extensively undergo glutaminolysis, utilizing glutamine as a carbon and nitrogen source [122, 123]. Upon entering the cell, glutamine is deaminated to form glutamate and ultimately α-ketoglutarate (α-KG), which serves as an anaplerotic substrate to replenish the TCA cycle (Figure 4). Located in the mitochondria, α-KGDH mediates the conversion of α-KG to succinyl-CoA. With α-KGDH being located at a critical junction that connects glutaminolysis with the TCA cycle, understanding the impact of thiamine supplementation on the functional contribution of α-KGDH to cancer metabolism is highly desirable. Unfortunately there are no reports to date describing changes in α-KGDH expression or activity in cancer.

In the oxidative direction, glutamine derived α-KG entering the TCA cycle is a key carbon backbone for the synthesis of amino acids and nucleotides [122]. Alternatively, glutamine-derived α-KG can undergo reductive decarboxylation through the TCA cycle, forming citrate for lipid biosynthesis [124, 125]. This reverse TCA carbon flow would bypass the requirement for α-KGDH activity, allowing the cell to utilize α-KG unabated by changes in thiamine availability. It is unclear how α-KGDH activity is regulated to allow α-KG to move in the reverse direction but has been described to be triggered during hypoxic stress [125, 126]. Normally, the activity of α-KGDH is stimulated by low concentrations of calcium and ADP and inhibited at high NADH and succinyl-CoA levels [127]. Reduced activity of α-KGDH has been observed during high oxidative stress that may provide a regulatory switch that allows for reverse TCA carbon flow during hypoxia [128]. Several studies have evaluated the effects of thiamine deficiency on expression and activity of α-KGDH with conflicting results. In human lymphoblasts, fibroblasts and neuroblastoma cells, no change in α-KGDH gene expression and activity were observed during thiamine deficiency [106]. In contrast, in vivo studies reported decreased α-KGDH activities in the neuronal tissues during thiamine deficiency [129, 130]. Therefore, clarification of α-KGDH regulation and what impact changes in thiamine availability have on activity and directional flux of the TCA cycle is greatly needed in cancer.

Branched chain alpha-keto acid dehydrogenase complex

Valine, isoleucine, and leucine are essential branched chain amino acids (BCAAs) that can serve as an energy source as well as precursors for amino acid and protein synthesis [131, 132]. The metabolism of BCAAs involves transamination to the α- keto acid followed by irreversible oxidative decarboxylation by the thiamine-dependent BCKDC to form an acyl-CoA derivative [132]. The continued catabolic breakdown of BCAA produces acetyl-CoA (from leucine) and succinyl-CoA (from valine and isoleucine) that enter the TCA cycle [132, 133]. BCKDC is a multi-component enzyme consisting of three subunits (E1, E2, and E3) and is located in the mitochondria [132]. Similar to PDH, BCKDC activity is regulated through reversible phosphorylation by branched-chain α- keto acid dehydrogenase kinase (BDK) and phosphatase (BDP) [134]. Loss-of-function mutations and BCKDC deficiencies are associated with accumulation of neurotoxic α- keto acids, referred to as maple syrup urine disease (MSUD) that is characterized by a sweet urine odor [135].

In hypermetabolic states such as exercise, sepsis, trauma, and cancer, the release of BCAAs from muscle protein provides a pool of amino acids for the synthesis of priority proteins and/or an important source of oxidative energy [136–138]. Many cancer patients experience involuntary weight loss termed cachexia, which is associated with a loss of skeletal muscle mass [139]. Several in vivo tumor models have demonstrated similar cachetic phenotypes. In Walker 256 carcinoma tumor-bearing rat models, an increase in protein degradation and leucine oxidation in skeletal muscle is observed [140, 141]. The increase in BCAA oxidation corresponds with an increase in BCKDC activity in the muscle tissue of Walker 256 and Morris hepatoma 5123 tumor-bearing animals [142–144]. Mediators of proteolysis and BCAA oxidation in muscle tissue of cancer patients may involve pro-inflammatory cytokines [142, 145]. Shiraki et al. assessed the effect of TNF-α administration on BCKDC activity in rat liver [146]. In TNF- ɑ- treated rats, BCKDC activity was higher than in control due to reduced BDK-mediated phosphorylation and inactivation. Downregulation of BDK mRNA by the glucocorticoid dexamethasone in rat hepatoma cell lines suggests hormonal regulation of BCAA metabolism and BCKDC activity [147]. A recent metabolomics profiling study of men after androgen deprivation therapy for prostate cancer demonstrated a decrease in the products of the BCKDC reaction, further demonstrating a role for hormone control of BCAA metabolism [148]. Interestingly, the thiamine cofactor TPP is a potent inhibitor (Ki = 4 μM) of BDK-mediated phosphorylation, allowing BCKDC to remain active [149]. High-dose thiamine supplementation increases BCKDC activity in thiamine-responsive MSUD patients [135, 150]. However, It is unknown what impact thiamine supplementation has on BCKDC activity and BCAA metabolism in cancer patients. Further research is required to establish if any relationship between thiamine supplementation with BCAA metabolism and cancer cachexia exists.

Conclusions

The alterations in thiamine homeostasis and increase in cancer cell proliferation with thiamine supplementation highlights a significant role for thiamine in cancer. Metabolic studies have provided strong evidence that cancer cells exploit thiamine-dependent enzymes and pathways for anabolic, proliferative, and survival purposes. Unfortunately, how thiamine supplementation impacts the metabolic phenotype of cancer cells is currently hypothetical and is an area of research greatly needed. Refinement of model systems will be absolutely essential in establishing the effects of increasing thiamine supplementation on cancer metabolism and proliferation. Common cell culture media contains super-physiological levels of thiamine that may obscure the importance of thiamine in cancer cell metabolism when using in vitro models. For instance, high glucose DMEM contains 10 μM thiamine, which is approximately 1,000 times greater than circulating plasma levels. Additionally, an organismal approach for understanding the role of thiamine in tumor metabolism will need to include the interplay with other nutrients, the tumor microenvironment, and tumor-stromal tissue demands for thiamine. Once connections between thiamine and cancer cell metabolism are established, new opportunities for therapeutic intervention and dietary modification to reduce disease progression in cancer patients will follow.

Author’s contributions

JZ conceived the review paper, and outline, and was responsible for revising and critically assessing the intellectual content. RS was responsible for critically reviewing the literature and drafting sections and figures within the manuscript. BH was responsible for critically reviewing the literature and drafting sections and figures within the manuscript. SY was responsible for critically reviewing the literature and drafting sections and figures within the manuscript. All authors read and approved the final manuscript.

Abbreviations

α-KG:

α-ketoglutarate

α-KGDH:

Alpha-ketoglutarate dehydrogenase

AThTP:

Adenosine thiamine triphosphate

BCAA:

Branched chain amino acid

BCKDC:

Branched chain alpha-keto acid dehydrogenase complex

BDP:

α-keto acid dehydrogenase phosphotase

BDK:

α-keto acid dehydrogenase kinase

DCA:

Dichloroacetate

DMEM:

Dulbecco’s modified eagle’s serum

DNC:

Deoxynucleotide carrier

DV:

Daily value

F6P:

Fructose 6-phosphate

5-FU:

5-fluorouracil

G3P:

Glyceraldehyde 3-phosphate

HIF-1α:

Hypoxia-inducible factor-1 alpha

hMEC:

Human mammary epithelial cell

1H-NMR:

Hydrogen-1-nuclear magnetic resonance

HPLC:

High performance liquid chromatography

LDHA:

Lactate dehydrogenase A

LLC:

Lewis lung carcinoma

LT:

Low thiamine

M2-PK:

M2 isoform of pyruvate kinase

MSUD:

Maple syrup urine disease

NADH:

Nicotinamide adenine dinucleotide

NAPDH:

Nicotinamide adenine dinucleotide phosphate-oxidase

NF:

Normal-fat

NT:

Normal thiamine

PDH:

Pyruvate dehydrogenase

PDK:

Pyruvate dehydrogenase kinase

PPP:

Phosphate pathway

RDI:

Recommended daily intake

ROS:

Reactive oxygen species

R5P:

Ribose 5-phosphate

TA:

transaldolase

TCA:

Tricarboxylic acid

TKT:

Transketolase

TKTΔ38:

38 amino acid deletion pseudo-TKTL1

TKTL:

TKT-like

TMP:

Thiamine monophosphate

TMPase:

Thiamine monophosphatase

TNF:

Tumor necrosis factor

TPC:

Thiamine pyrophosphate carrier

TPK1:

Thiamine pyrophosphokinase-1

TPP:

Thiamine pyrophosphate

TPPase:

Thiamine pyrophosphatase

TTP:

Thiamine triphosphate

USDA:

United States Department of Agriculture

X5P:

Xylulose 5-phsophate.

References

  1. Gangolf M, Czerniecki J, Radermecker M, Detry O, Nisolle M, Jouan C, Martin D, Chantraine F, Lakaye B, Wins P, Grisar T, Bettendorff L: Thiamine status in humans and content of phosphorylated thiamine derivatives in biopsies and cultured cells. PLoS One. 2010, 5: e13616-10.1371/journal.pone.0013616.

    PubMed Central  PubMed  Google Scholar 

  2. Institute of Medicine (US) Standing Committee on the Scientific Evaluation of Dietary Reference Intakes and its Panel on Folate, Other B Vitamins, and Choline: Dietary Reference Intakes for Thiamin, Riboflavin, Niacin, Vitamin B6, Folate, Vitamin B12, Pantothenic Acid, Biotin, and Choline. 1998, Washington, DC: National Academies Press (US)

    Google Scholar 

  3. Ganapathy V, Smith SB, Prasad PD: SLC19: the folate/thiamine transporter family. Pfluegers Arch. 2004, 447: 641-646. 10.1007/s00424-003-1068-1.

    CAS  Google Scholar 

  4. Rajgopal A, Edmondnson A, Goldman ID, Zhao R: SLC19A3 encodes a second thiamine transporter ThTr2. Biochim Biophys Acta. 2001, 1537: 175-178. 10.1016/S0925-4439(01)00073-4.

    CAS  PubMed  Google Scholar 

  5. Subramanian VS, Marchant JS, Parker I, Said HM: Cell biology of the human thiamine transporter-1 (hTHTR1). J Biol Chem. 2003, 278: 3976-3984. 10.1074/jbc.M210717200.

    CAS  PubMed  Google Scholar 

  6. Dutta B, Huang W, Molero M, Kekuda R, Leibach FH, Devoe LD, Ganapathy V, Prasad PD: Cloning of the human thiamine transporter, a member of the folate transporter family. J Biol Chem. 1999, 274: 31925-31929. 10.1074/jbc.274.45.31925.

    CAS  PubMed  Google Scholar 

  7. Said HM, Balamurugan K, Subramanian VS, Marchant JS: Expression and functional contribution of hTHTR-2 in thiamin absorption in human intestine. Am J Physiol. 2004, 286: G491-G498.

    CAS  Google Scholar 

  8. Hoyumpa AM, Strickland R, Sheehan JJ, Yarborough G, Nichols S: Dual system of intestinal thiamine transport in humans. J Lab Clin Med. 1982, 99: 701-708.

    CAS  PubMed  Google Scholar 

  9. Zielinska-Dawidziak M, Grajek K, Olejnik A, Czaczyk K, Grajek W: Transport of high concentration of thiamin, riboflavin and pyridoxine across intestinal epithelial cells Caco-2. J Nutr Sci Vitaminol. 2008, 54: 423-429. 10.3177/jnsv.54.423.

    CAS  PubMed  Google Scholar 

  10. Lemos C, Faria A, Meireles M, Martel F, Monteiro R, Calhau C: Thiamine is a substrate of organic cation transporters in Caco-2 cells. Eur J Pharmacol. 2012, 682: 37-42. 10.1016/j.ejphar.2012.02.028.

    CAS  PubMed  Google Scholar 

  11. Nabokina SM, Said HM: A high-affinity and specific carrier-mediated mechanism for uptake of thiamine pyrophosphate by human colonic epithelial cells. Am J Physiol Gastrointest Liver Physiol. 2012, 303: G389-395. 10.1152/ajpgi.00151.2012.

    PubMed Central  PubMed  Google Scholar 

  12. Nosaka K, Onozuka M, Kakazu N, Hibi S, Nishimura H, Nishino H, Abe T: Isolation and characterization of a human thiamine pyrophosphokinase cDNA. Biochim Biophys Acta. 2001, 1517: 293-297. 10.1016/S0167-4781(00)00247-5.

    CAS  PubMed  Google Scholar 

  13. Bettendorff L: Thiamine homeostasis in neuroblastoma cells. Neurochem Int. 1995, 26: 295-302. 10.1016/0197-0186(94)00123-C.

    CAS  PubMed  Google Scholar 

  14. Bellyei S, Szigeti A, Boronkai A, Szabo Z, Bene J, Janaky T, Barna L, Sipos K, Minik O, Kravjak A, Ohmacht R, Melegh B, Zavodszky P, Than GN, Sumegi B, Bohn H, Than NG: Cloning, sequencing, structural and molecular biological characterization of placental protein 20 (PP20)/human thiamin pyrophosphokinase (hTPK). Placenta. 2005, 26: 34-46. 10.1016/j.placenta.2004.03.008.

    CAS  PubMed  Google Scholar 

  15. Rindi G, Laforenza U: Thiamine intestinal transport and related issues: recent aspects. Proc Soc Exp Biol Med. 2000, 224: 246-255. 10.1046/j.1525-1373.2000.22428.x.

    CAS  PubMed  Google Scholar 

  16. Zhao R, Gao F, Goldman ID: Reduced folate carrier transports thiamine monophosphate: an alternative route for thiamine delivery into mammalian cells. Am J Physiol Cell Physiol. 2002, 282: C1512-1517. 10.1152/ajpcell.00547.2001.

    CAS  PubMed  Google Scholar 

  17. Zhao R, Gao F, Wang Y, Diaz GA, Gelb BD, Goldman ID: Impact of the reduced folate carrier on the accumulation of active thiamin metabolites in murine leukemia cells. J Biol Chem. 2001, 276: 1114-1118. 10.1074/jbc.M007919200.

    CAS  PubMed  Google Scholar 

  18. Lindhurst MJ, Fiermonte G, Song S, Struys E, De Leonardis F, Schwartzberg PL, Chen A, Castegna A, Verhoeven N, Mathews CK, Palmieri F, Biesecker LG: Knockout of Slc25a19 causes mitochondrial thiamine pyrophosphate depletion, embryonic lethality, CNS malformations, and anemia. Proc Natl Acad Sci. 2006, 103: 15927-15932. 10.1073/pnas.0607661103.

    PubMed Central  CAS  PubMed  Google Scholar 

  19. Kang J, Samuels DC: The evidence that the DNC (SLC25A19) is not the mitochondrial deoxyribonucleotide carrier. Mitochondrion. 2008, 8: 103-108. 10.1016/j.mito.2008.01.001.

    CAS  PubMed  Google Scholar 

  20. Liu S, Huang H, Lu X, Golinski M, Comesse S, Watt D, Grossman RB, Moscow JA: Down-regulation of thiamine transporter THTR2 gene expression in breast cancer and is association with resistance to apoptosis. Mol Cancer Res. 2003, 1: 665-673.

    CAS  PubMed  Google Scholar 

  21. Liu X, Lam EK-Y, Wang X, Zhang J-B, Cheng Y-Y, Lam YW, Ng EK-O, Yu J, Chan FK-L, Jin H-C, Sung JJ-Y: Promoter hypermethylation mediates downregulation of thiamine receptor SLC19A3 in gastric cancer. Tumor Biol. 2009, 30: 242-248. 10.1159/000243767.

    Google Scholar 

  22. Ikehata M, Ueda K, Iwakawa S: Different involvement of DNA methylation and histone deacetylation in the expression of solute-carrier transporters in 4 colon cancer cell lines. Biol Pharm Bull. 2012, 35: 301-307. 10.1248/bpb.35.301.

    CAS  PubMed  Google Scholar 

  23. Ng EK, Leung CP, Shin VY, Wong CL, Ma ES, Jin HC, Chu KM, Kwong A: Quantitative analysis and diagnostic significance of methylated SLC19A3 DNA in the plasma of breast and gastric cancer patients. PLoS One. 2011, 6: e22233-10.1371/journal.pone.0022233.

    PubMed Central  CAS  PubMed  Google Scholar 

  24. Zastre JA, Hanberry BS, Sweet RL, McGinnis AC, Venuti KR, Bartlett MG, Govindarajan R: Up-regulation of vitamin B1 homeostasis genes in breast cancer. J Nutr Biochem. 2013, 10.1016/j.jnutbio.2013.02.002. [Epub ahead of print]

    Google Scholar 

  25. Liu S, Stromberg A, Tai H-H, Moscow JA: Thiamine transporter gene expression and exogenous thiamine modulate the expression of genes involved in drug and prostaglandin metabolism in breast cancer cells. Mol Cancer Res. 2004, 2: 477-487.

    CAS  PubMed  Google Scholar 

  26. Sweet R, Paul A, Zastre J: Hypoxia induced upregulation and function of the thiamine transporter, SLC19A3 in a breast cancer cell line. Cancer Biol Ther. 2010, 10: 1101-1111. 10.4161/cbt.10.11.13444.

    CAS  PubMed  Google Scholar 

  27. Hee SB, Hyuk CS, Young CE, Min-Jeong S, Ki-Chul H, Keun CH, Hyung CJ, Yangsoo J: Thiamine attenuates hypoxia-induced cell death in cultured neonatal rat cardiomyocytes. Mol Cells. 2004, 18: 133-140.

    Google Scholar 

  28. Chandel NS, Maltepe E, Goldwasser E, Mathieu CE, Simon MC, Schumacker PT: Mitochondrial reactive oxygen species trigger hypoxia-induced transcription. Proc Natl Acad Sci. 1998, 95: 11715-11720. 10.1073/pnas.95.20.11715.

    PubMed Central  CAS  PubMed  Google Scholar 

  29. Liu L, Wise DR, Diehl JA, Simon MC: Hypoxic reactive oxygen species regulate the integrated stress response and cell survival. J Biol Chem. 2008, 283: 31153-31162. 10.1074/jbc.M805056200.

    PubMed Central  CAS  PubMed  Google Scholar 

  30. Guzy RD, Hoyos B, Robin E, Chen H, Liu L, Mansfield KD, Simon MC, Hammerling U, Schumacker PT: Mitochondrial complex III is required for hypoxia-induced ROS production and cellular oxygen sensing. Cell Metab. 2005, 1: 401-408. 10.1016/j.cmet.2005.05.001.

    CAS  PubMed  Google Scholar 

  31. Trachootham D, Alexandre J, Huang P: Targeting cancer cells by ROS-mediated mechanisms: a radical therapeutic approach?. Nat Rev Drug Discov. 2009, 8: 579-591. 10.1038/nrd2803.

    CAS  PubMed  Google Scholar 

  32. Simon HU, Haj-Yehia A, Levi-Schaffer F: Role of reactive oxygen species (ROS) in apoptosis induction. Apoptosis. 2000, 5: 415-418. 10.1023/A:1009616228304.

    CAS  PubMed  Google Scholar 

  33. Lukienko PI, Mel’nichenko NG, Zverinskii IV, Zabrodskaya SV: Antioxidant properties of thiamine. Bull Exp Biol Med. 2000, 130: 874-876.

    CAS  PubMed  Google Scholar 

  34. Schmid U, Stopper H, Heidland A, Schupp N: Benfotiamine exhibits direct antioxidative capacity and prevents induction of DNA damage in vitro. Diabetes Metab Res Rev. 2008, 24: 371-377. 10.1002/dmrr.860.

    CAS  PubMed  Google Scholar 

  35. Martin PR, Singleton CK, Hiller-Sturmhofel S: The role of thiamine deficiency in alcoholic brain disease. Alcohol Res Health. 2003, 27: 134-142.

    PubMed  Google Scholar 

  36. Boros LG: Population thiamine status and varying cancer rates between western, Asian and African countries. Anticancer Res. 2000, 20: 2245-2248.

    CAS  PubMed  Google Scholar 

  37. Kaul L, Heshmat MY, Kovi J, Jackson MA, Jackson AG, Jones GW, Edson M, Enterline JP, Worrell RG, Perry SL: The role of diet in prostate cancer. Nutr Cancer. 1987, 9: 123-128. 10.1080/01635588709513919.

    CAS  PubMed  Google Scholar 

  38. Kabat GC, Miller AB, Jain M, Rohan TE: Dietary intake of selected B vitamins in relation to risk of major cancers in women. Br J Cancer. 2008, 99: 816-821. 10.1038/sj.bjc.6604540.

    PubMed Central  CAS  PubMed  Google Scholar 

  39. Bruce WR, Furrer R, Shangari N, O’Brien PJ, Medline A, Wang Y: Marginal dietary thiamin deficiency induces the formation of colonic aberrant crypt foci (ACF) in rats. Cancer Lett. 2003, 202: 125-129. 10.1016/j.canlet.2003.08.005.

    CAS  PubMed  Google Scholar 

  40. Lee BY, Yanamandra K, Bocchini JA: Thiamin deficiency: a possible major cause of some tumors?. Oncol Rep. 2005, 14: 1589-1592.

    CAS  PubMed  Google Scholar 

  41. Chamberlain BR, Buttery JE, Pannall PR: A stable reagent mixture for the whole blood transketolase assay. Ann Clin Biochem. 1996, 33: 352-354.

    CAS  PubMed  Google Scholar 

  42. Basu TK, Dickerson JWT: The thiamin status of early cancer patients with particular reference to those with breast and bronchial carcinomas. Oncol. 1976, 33: 250-252. 10.1159/000225157.

    CAS  Google Scholar 

  43. Basu TK, Dickerson JW, Raven RW, Williams DC: The thiamine status of patients with cancer as determined by the red cell transketolase activity. Int J Vitam Nutr Res. 1974, 44: 53-58.

    CAS  PubMed  Google Scholar 

  44. van Zaanen HC, van der Lelie J: Thiamine deficiency in hematologic malignant tumors. Cancer. 1992, 69: 1710-1713. 10.1002/1097-0142(19920401)69:7<1710::AID-CNCR2820690711>3.0.CO;2-D.

    CAS  PubMed  Google Scholar 

  45. Seligmann H, Levi R, Konijn AM, Prokocimer M: Thiamine deficiency in patients with B-chronic lymphocytic leukaemia: a pilot study. Postgrad Med J. 2001, 77: 582-585. 10.1136/pmj.77.911.582.

    PubMed Central  CAS  PubMed  Google Scholar 

  46. Tsao S-M, Yin M-C, Liu W-H: Oxidant stress and B vitamins status in patients with non-small cell lung cancer. Nutr Cancer. 2007, 59: 8-13. 10.1080/01635580701365043.

    CAS  PubMed  Google Scholar 

  47. Trebukhina RV, Ostrovsky YM, Shapot VS, Mikhaltsevich GN, Tumanov VN: Turnover of [14C]thiamin and activities of thiamin pyrophosphate-dependent enzymes in tissues of mice with Ehrlich ascites carcinoma. Nutr Cancer. 1984, 6: 260-273.

    CAS  PubMed  Google Scholar 

  48. Trebukhina RV, Ostrovsky Yu M, Shapot VS, Petushok VG, Velichko MG, Tumanov VN, Mikhaltsevich GN: Thiamine metabolism in the liver of mice with Ehrlich ascites carcinoma. Neoplasma. 1982, 29: 257-268.

    CAS  PubMed  Google Scholar 

  49. Baker H, Frank O, Chen T, Feingold S, DeAngelis B, Baker ER: Elevated vitamin levels in colon adenocarcinoma as compared with metastatic liver adenocarcinoma from colon primary and normal adjacent tissue. Cancer. 1981, 47: 2883-2886. 10.1002/1097-0142(19810615)47:12<2883::AID-CNCR2820471222>3.0.CO;2-I.

    CAS  PubMed  Google Scholar 

  50. Aksoy M, Basu TK, Brient J, Dickerson JW: Thiamin status of patients treated with drug combinations containing 5-fluorouracil. Eur J Cancer. 1980, 16: 1041-1045.

    CAS  PubMed  Google Scholar 

  51. Buesa JM, Garcia-Teijido P, Losa R, Fra J: Treatment of ifosfamide encephalopathy with intravenous thiamin. Clin Cancer Res. 2003, 9: 4636-4637.

    CAS  PubMed  Google Scholar 

  52. Ames B, Lewis LD, Chaffee S, Kim J, Morse R: Ifosfamide-induced encephalopathy and movement disorder. Pediatr Blood Cancer. 2010, 54: 624-626.

    PubMed  Google Scholar 

  53. Hamadani M, Awan F: Role of thiamine in managing ifosfamide-induced encephalopathy. J Oncol Pharm Pract. 2006, 12: 237-239. 10.1177/1078155206073553.

    CAS  PubMed  Google Scholar 

  54. Lombardi G, Zustovich F, Nicoletto MO, Donach M, Pastorelli D: Important role of thiamine in preventing ifosfamide-induced encephalopathy. J Oncol Pharm Pract. 2010, 16: 135-136. 10.1177/1078155209342134.

    CAS  PubMed  Google Scholar 

  55. Losa R, Sierra MI, Fernández A, Blanco D, Buesa JM: Determination of thiamine and its phosphorylated forms in human plasma, erythrocytes and urine by HPLC and fluorescence detection: a preliminary study on cancer patients. J Pharm Biomed Anal. 2005, 37: 1025-1029. 10.1016/j.jpba.2004.08.038.

    CAS  PubMed  Google Scholar 

  56. Heier MS, Dornish JM: Effect of the fluoropyrimidines 5-fluorouracil and doxifluridine on cellular uptake of thiamin. Anticancer Res. 1989, 9: 1073-1077.

    CAS  PubMed  Google Scholar 

  57. Basu TK, Aksoy M, Dickerson JW: Effects of 5-fluorouracil on the thiamin status of adult female rats. Chemother. 1979, 25: 70-76. 10.1159/000237825.

    CAS  Google Scholar 

  58. Comin-Anduix B, Boren J, Martinez S, Moro C, Centelles JJ, Trebukhina R, Petushok N, Lee W-NP, Boros LG, Cascante M: The effect of thiamine supplementation on tumour proliferation. A metabolic control analysis study. Eur J Biochem. 2001, 268: 4177-4182. 10.1046/j.1432-1327.2001.02329.x.

    CAS  PubMed  Google Scholar 

  59. Daily A, Liu S, Bhatnagar S, Karabakhtsian RG, Moscow JA: Low-thiamine diet increases mammary tumor latency in FVB/N-Tg(MMTVneu) mice. Int J Vitam Nutr Res. 2012, 82: 298-302. 10.1024/0300-9831/a000122.

    CAS  PubMed  Google Scholar 

  60. Liu S, Monks NR, Hanes JW, Begley TP, Yu H, Moscow JA: Sensitivity of breast cancer cell lines to recombinant thiaminase I. Cancer Chemother Pharmacol. 2010, 66: 171-179. 10.1007/s00280-009-1148-9.

    CAS  PubMed  Google Scholar 

  61. Daily A, Liu S, Bae Y, Bhatnagar S, Moscow JA: Linear chain PEGylated recombinant Bacillus thiaminolyticus thiaminase I enzyme has growth inhibitory activity against lymphoid leukemia cell lines. Mol Cancer Ther. 2011, 10: 1563-1570. 10.1158/1535-7163.MCT-11-0003.

    CAS  PubMed  Google Scholar 

  62. Boros LG, Puigjaner J, Cascante M, Lee W-NP, Brandes JL, Bassilian S, Yusuf FI, Williams RD, Muscarella P, Melvin WS, Schirmer WJ: Oxythiamine and dehydroepiandrosterone inhibit the nonoxidative synthesis of ribose and tumor cell proliferation. Cancer Res. 1997, 57: 4242-4248.

    CAS  PubMed  Google Scholar 

  63. Rais B, Comin B, Puigjaner J, Brandes JL, Creppy E, Saboureau D, Ennamany R, Lee W-NP, Boros LG, Cascante M: Oxythiamin and dehydroepiandrosterone induce a G1 phase cycle arrest in Ehrlich’s tumor cells through inhibition of the pentose cycle. FEBS Lett. 1999, 456: 113-118. 10.1016/S0014-5793(99)00924-2.

    CAS  PubMed  Google Scholar 

  64. Ramos-Montoya A, Lee WN, Bassilian S, Lim S, Trebukhina RV, Kazhyna MV, Ciudad CJ, Noe V, Centelles JJ, Cascante M: Pentose phosphate cycle oxidative and nonoxidative balance: A new vulnerable target for overcoming drug resistance in cancer. Int J Cancer. 2006, 119: 2733-2741. 10.1002/ijc.22227.

    CAS  PubMed  Google Scholar 

  65. Chornyy S, Parkhomenko J, Chorna N: Thiamine deficiency caused by thiamine antagonists triggers upregulation of apoptosis inducing factor gene expression and leads to caspase 3-mediated apoptosis in neuronally differentiated rat PC-12 cells. Acta Biochim Pol. 2007, 54: 315-322.

    CAS  PubMed  Google Scholar 

  66. Yang CM, Liu YZ, Liao JW, Hu ML: The in vitro and in vivo anti-metastatic efficacy of oxythiamine and the possible mechanisms of action. Clin Exp Metastasis. 2010, 27: 341-349. 10.1007/s10585-010-9331-2.

    CAS  PubMed  Google Scholar 

  67. Boros LG, Torday JS, Lim S, Bassilian S, Cascante M, Lee W-NP: Transforming growth factor beta 2 promotes glucose carbon incorporation into nucleic acid ribose through the nonoxidative pentose cycle in lung epithelial carcinoma cells. Cancer Res. 2000, 60: 1183-1185.

    CAS  PubMed  Google Scholar 

  68. Christofk HR, Vander Heiden MG, Harris MH, Ramanathan A, Gerszten RE, Wei R, Fleming MD, Schreiber SL, Cantley LC: The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth. Nat. 2008, 452: 230-233. 10.1038/nature06734.

    CAS  Google Scholar 

  69. Mazurek S: Pyruvate kinase type M2: a key regulator of the metabolic budget system in tumor cells. Int J Biochem Cell Biol. 2011, 43: 969-980. 10.1016/j.biocel.2010.02.005.

    CAS  PubMed  Google Scholar 

  70. Mazurek S, Boschek CB, Hugo F, Eigenbrodt E: Pyruvate kinase type M2 and its role in tumor growth and spreading. Semin Cancer Biol. 2005, 15: 300-308. 10.1016/j.semcancer.2005.04.009.

    CAS  PubMed  Google Scholar 

  71. Eigenbrodt E, Basenau D, Holthusen S, Mazurek S, Fischer G: Quantification of tumor type M2 pyruvate kinase (Tu M2-PK) in human carcinomas. Anticancer Res. 1997, 17: 3153-3156.

    CAS  PubMed  Google Scholar 

  72. Schneider J, Morr H, Velcovsky HG, Weisse G, Eigenbrodt E: Quantitative detection of tumor M2-pyruvate kinase in plasma of patients with lung cancer in comparison to other lung diseases. Cancer Detect Prev. 2000, 24: 531-535.

    CAS  PubMed  Google Scholar 

  73. Hathurusinghe HR, Goonetilleke KS, Siriwardena AK: Current status of tumor M2 pyruvate kinase (tumor M2-PK) as a biomarker of gastrointestinal malignancy. Ann Surg Oncol. 2007, 14: 2714-2720. 10.1245/s10434-007-9481-x.

    PubMed  Google Scholar 

  74. Mazurek S, Boschek CB, Eigenbrodt E: The role of phosphometabolites in cell proliferation, energy metabolism, and tumor therapy. J Bioenerg Biomembr. 1997, 29: 315-330. 10.1023/A:1022490512705.

    CAS  PubMed  Google Scholar 

  75. Chinnaiyan P, Kensicki E, Bloom G, Prabhu A, Sarcar B, Kahali S, Eschrich S, Qu X, Forsyth P, Gillies R: The metabolomic signature of malignant glioma reflects accelerated anabolic metabolism. Cancer Res. 2012, 72: 5878-5888. 10.1158/0008-5472.CAN-12-1572-T.

    CAS  PubMed  Google Scholar 

  76. Zhao F, Mancuso A, Bui TV, Tong X, Gruber JJ, Swider CR, Sanchez PV, Lum JJ, Sayed N, Melo JV, Perl AE, Carroll M, Tuttle SW, Thompson CB: Imatinib resistance associated with BCR-ABL upregulation is dependent on HIF-1alpha -induced metabolic reprograming. Oncog. 2010, 29: 2962-2972. 10.1038/onc.2010.67.

    CAS  Google Scholar 

  77. Serkova N, Boros LG: Detection of resistance to imatinib by metabolic profiling: clinical and drug development implications. Am J Pharmacogenomics. 2005, 5: 293-302. 10.2165/00129785-200505050-00002.

    CAS  PubMed  Google Scholar 

  78. Langbein S, Zerilli M, zur Hausen A, Staiger W, Rensch-Boschert K, Lukan N, Popa J, Ternullo MP, Steidler A, Weiss C, Grobholz R, Willeke F, Alken P, Stassi G, Schubert P, Coy JF: Expression of transketolase TKTL1 predicts colon and urothelial cancer patient survival: Warburg effect reinterpreted. Br J Cancer. 2006, 94: 578-585. 10.1038/sj.bjc.6602962.

    PubMed Central  CAS  PubMed  Google Scholar 

  79. Diaz-Moralli S, Tarrado-Castellarnau M, Alenda C, Castells A, Cascante M: Transketolase-like 1 expression is modulated during colorectal cancer progression and metastasis formation. PLoS One. 2011, 6: e25323-10.1371/journal.pone.0025323.

    PubMed Central  CAS  PubMed  Google Scholar 

  80. Staiger WI, Coy JF, Grobholz R, Hofheinz RD, Lukan N, Post S, Schwarzbach MH, Willeke F: Expression of the mutated transketolase TKTL1, a molecular marker in gastric cancer. Oncol Rep. 2006, 16: 657-661.

    CAS  PubMed  Google Scholar 

  81. Foeldi M, Stickeler E, Bau L, Kretz O, Watermann D, Gitsch G, Kayser G, Zur Hausen A, Coy JF: Transketolase protein TKTL1 overexpression: a potential biomarker and therapeutic target in breast cancer. Oncol Rep. 2007, 17: 841-845.

    CAS  Google Scholar 

  82. Krockenberger M, Engel JB, Schmidt M, Kohrenhagen N, Hausler SF, Dombrowski Y, Kapp M, Dietl J, Honig A: Expression of transketolase-like 1 protein (TKTL1) in human endometrial cancer. Anticancer Res. 2010, 30: 1653-1659.

    CAS  PubMed  Google Scholar 

  83. Schultz H, Kaehler D, Branscheid D, Vollmer E, Zabel P, Goldmann T: TKTL1 is overexpressed in a large portion of non-small cell lung cancer specimens. Diagn Pathol. 2008, 3: 35-10.1186/1746-1596-3-35.

    PubMed Central  PubMed  Google Scholar 

  84. Krockenberger M, Honig A, Rieger L, Coy JF, Sutterlin M, Kapp M, Horn E, Dietl J, Kammerer U: Transketolase-like 1 expression correlates with subtypes of ovarian cancer and the presence of distant metastases. Int J Gynecol Cancer. 2007, 17: 101-106. 10.1111/j.1525-1438.2007.00799.x.

    CAS  PubMed  Google Scholar 

  85. Volker HU, Scheich M, Schmausser B, Kammerer U, Eck M: Overexpression of transketolase TKTL1 is associated with shorter survival in laryngeal squamous cell carcinomas. Eur Arch Otorhinolaryngol. 2007, 264: 1431-1436. 10.1007/s00405-007-0394-x.

    PubMed  Google Scholar 

  86. Schmidt M, Voelker H-U, Kapp M, Krockenberger M, Dietl J, Kammerer U: Glycolytic phenotype in breast cancer: activation of Akt, up-regulation of GLUT1, TKTL1 and down-regulation of M2PK. J Cancer Res Clin Oncol. 2010, 136: 219-225. 10.1007/s00432-009-0652-y.

    CAS  PubMed  Google Scholar 

  87. Schwaab J, Horisberger K, Strobel P, Bohn B, Gencer D, Kahler G, Kienle P, Post S, Wenz F, Hofmann WK, Hofheinz RD, Erben P: Expression of Transketolase like gene 1 (TKTL1) predicts disease-free survival in patients with locally advanced rectal cancer receiving neoadjuvant chemoradiotherapy. BMC Cancer. 2011, 11: 363-10.1186/1471-2407-11-363.

    PubMed Central  CAS  PubMed  Google Scholar 

  88. Hu L-H, Yang J-H, Zhang D-T, Zhang S, Wang L, Cai P-C, Zheng J-F, Huang J-S: The TKTL1 gene influences total transketolase activity and cell proliferation in human colon cancer LoVo cells. Anticancer Drugs. 2007, 18: 427-433. 10.1097/CAD.0b013e328013d99e.

    CAS  PubMed  Google Scholar 

  89. Zhang S, Yue JX, Yang JH, Cai PC, Kong WJ: Overexpression of transketolase protein TKTL1 is associated with occurrence and progression in nasopharyngeal carcinoma. Cancer Biol Ther. 2008, 7: 517-522. 10.4161/cbt.7.4.5479.

    CAS  PubMed  Google Scholar 

  90. Chen H, Yue J-X, Yang S-H, Ding H, Zhao R-W, Zhang S: Overexpression of transketolase-like gene 1 is associated with cell proliferation in uterine cervix cancer. J Exp Clin Cancer Res. 2009, 28: 43-10.1186/1756-9966-28-43.

    PubMed Central  CAS  PubMed  Google Scholar 

  91. Xu X, Zur Hausen A, Coy JF, Lochelt M: Transketolase-like protein 1 (TKTL1) is required for rapid cell growth and full viability of human tumor cells. Int J Cancer. 2009, 124: 1330-1337. 10.1002/ijc.24078.

    CAS  PubMed  Google Scholar 

  92. Zhang S, Yang J-H, Guo C-K, Cai P-c: Gene silencing of TKTL1 by RNAi inhibits cell proliferation in human hepatoma cells. Cancer Lett. 2007, 253: 108-114. 10.1016/j.canlet.2007.01.010.

    CAS  PubMed  Google Scholar 

  93. Yuan W, Wu S, Guo J, Chen Z, Ge J, Yang P, Hu B, Chen Z: Silencing of TKTL1 by siRNA inhibits proliferation of human gastric cancer cells in vitro and in vivo. Cancer Biol Ther. 2010, 9: 710-716. 10.4161/cbt.9.9.11431.

    CAS  PubMed  Google Scholar 

  94. Sun W, Liu Y, Glazer CA, Shao C, Bhan S, Demokan S, Zhao M, Rudek MA, Ha PK, Califano JA: TKTL1 is activated by promoter hypomethylation and contributes to head and neck squamous cell carcinoma carcinogenesis through increased aerobic glycolysis and HIF1alpha stabilization. Clin Cancer Res. 2010, 16: 857-866. 10.1158/1078-0432.CCR-09-2604.

    PubMed Central  CAS  PubMed  Google Scholar 

  95. Anastasiou D, Poulogiannis G, Asara JM, Boxer MB, Jiang JK, Shen M, Bellinger G, Sasaki AT, Locasale JW, Auld DS, Thomas CJ, Vander Heiden MG, Cantley LC: Inhibition of pyruvate kinase M2 by reactive oxygen species contributes to cellular antioxidant responses. Sci. 2011, 334: 1278-1283. 10.1126/science.1211485.

    CAS  Google Scholar 

  96. Wanka C, Steinbach JP, Rieger J: Tp53-induced glycolysis and apoptosis regulator (TIGAR) protects glioma cells from starvation-induced cell death by up-regulating respiration and improving cellular redox homeostasis. J Biol Chem. 2012, 287: 33436-33446. 10.1074/jbc.M112.384578.

    PubMed Central  CAS  PubMed  Google Scholar 

  97. Mitschke L, Parthier C, Schroder-Tittmann K, Coy J, Ludtke S, Tittmann K: The crystal structure of human transketolase and new insights into its mode of action. J Biol Chem. 2010, 285: 31559-31570. 10.1074/jbc.M110.149955.

    PubMed Central  CAS  PubMed  Google Scholar 

  98. Maslova AO, Meshalkina LE, Kochetov GA: Computer modeling of transketolase-like protein, TKTL1, a marker of certain tumor tissues. Biochem. 2012, 77: 296-299.

    CAS  Google Scholar 

  99. Schneider S, Ludtke S, Schroder-Tittmann K, Wechsler C, Meyer D, Tittmann K: A delta38 deletion variant of human transketolase as a model of transketolase-like protein 1 exhibits no enzymatic activity. PLoS One. 2012, 7: e48321-10.1371/journal.pone.0048321.

    PubMed Central  CAS  PubMed  Google Scholar 

  100. Meshalkina LE, Drutsa VL, Koroleva ON, Solovjeva ON, Kochetov GA: Is transketolase-like protein, TKTL1, transketolase?. Biochim Biophys Acta. 2013, 1832: 387-390. 10.1016/j.bbadis.2012.12.004.

    CAS  PubMed  Google Scholar 

  101. Brownlee M: Biochemistry and molecular cell biology of diabetic complications. Nat. 2001, 414: 813-820. 10.1038/414813a.

    CAS  Google Scholar 

  102. Babaei-Jadidi R, Karachalias N, Ahmed N, Battah S, Thornalley PJ: Prevention of incipient diabetic nephropathy by high-dose thiamine and benfotiamine. Diab. 2003, 52: 2110-2120. 10.2337/diabetes.52.8.2110.

    CAS  Google Scholar 

  103. Hammes HP, Du X, Edelstein D, Taguchi T, Matsumura T, Ju Q, Lin J, Bierhaus A, Nawroth P, Hannak D, Neumaier M, Bergfeld R, Giardino I, Brownlee M: Benfotiamine blocks three major pathways of hyperglycemic damage and prevents experimental diabetic retinopathy. Nat Med. 2003, 9: 294-299. 10.1038/nm834.

    CAS  PubMed  Google Scholar 

  104. Rabbani N, Thornalley PJ: Emerging role of thiamine therapy for prevention and treatment of early-stage diabetic nephropathy. Diab Obes Metab. 2011, 13: 577-583. 10.1111/j.1463-1326.2011.01384.x.

    CAS  Google Scholar 

  105. Berrone E, Beltramo E, Solimine C, Ape AU, Porta M: Regulation of intracellular glucose and polyol pathway by thiamine and benfotiamine in vascular cells cultured in high glucose. J Biol Chem. 2006, 281: 9307-9313.

    CAS  PubMed  Google Scholar 

  106. Pekovich SR, Martin PR, Singleton CK: Thiamine deficiency decreases steady-state transketolase and pyruvate dehydrogenase but not alpha-ketoglutarate dehydrogenase mRNA levels in three human cell types. J Nutr. 1998, 128: 683-687.

    CAS  PubMed  Google Scholar 

  107. Patel MS, Roche TE: Molecular biology and biochemistry of pyruvate dehydrogenase complexes. FASEB J. 1990, 4: 3224-3233.

    CAS  PubMed  Google Scholar 

  108. Korotchkina LG, Patel MS: Site specificity of four pyruvate dehydrogenase kinase isoenzymes toward the three phosphorylation sites of human pyruvate dehydrogenase. J Biol Chem. 2001, 276: 37223-37229. 10.1074/jbc.M103069200.

    CAS  PubMed  Google Scholar 

  109. Holness MJ, Sugden MC: Regulation of pyruvate dehydrogenase complex activity by reversible phosphorylation. Biochem Soc Trans. 2003, 31: 1143-1151. 10.1042/BST0311143.

    CAS  PubMed  Google Scholar 

  110. Kolobova E, Tuganova A, Boulatnikov I, Popov KM: Regulation of pyruvate dehydrogenase activity through phosphorylation at multiple sites. Biochem J. 2001, 358: 69-77. 10.1042/0264-6021:3580069.

    PubMed Central  CAS  PubMed  Google Scholar 

  111. Lu CW, Lin SC, Chien CW, Lin SC, Lee CT, Lin BW, Lee JC, Tsai SJ: Overexpression of pyruvate dehydrogenase kinase 3 increases drug resistance and early recurrence in colon cancer. Am J Pathol. 2011, 179: 1405-1414. 10.1016/j.ajpath.2011.05.050.

    PubMed Central  CAS  PubMed  Google Scholar 

  112. Hur H, Xuan Y, Kim YB, Lee G, Shim W, Yun J, Ham IH, Han SU: Expression of pyruvate dehydrogenase kinase-1 in gastric cancer as a potential therapeutic target. Int J Oncol. 2013, 42: 44-54.

    PubMed Central  CAS  PubMed  Google Scholar 

  113. Baumunk D, Reichelt U, Hildebrandt J, Krause H, Ebbing J, Cash H, Miller K, Schostak M, Weikert S: Expression parameters of the metabolic pathway genes pyruvate dehydrogenase kinase-1 (PDK-1) and DJ-1/PARK7 in renal cell carcinoma (RCC). World J Urol. 2012, 1-6. 10.1007/s00345-012-0874-5.

    Google Scholar 

  114. Koukourakis MI, Giatromanolaki A, Sivridis E, Gatter KC, Harris AL: Pyruvate dehydrogenase and pyruvate dehydrogenase kinase expression in non small cell lung cancer and tumor-associated stroma. Neoplasia. 2005, 7: 1-6. 10.1593/neo.04373.

    PubMed Central  CAS  PubMed  Google Scholar 

  115. McFate T, Mohyeldin A, Lu H, Thakar J, Henriques J, Halim ND, Wu H, Schell MJ, Tsang TM, Teahan O, Zhou S, Califano JA, Jeoung NH, Harris RA, Verma A: Pyruvate dehydrogenase complex activity controls metabolic and malignant phenotype in cancer cells. J Biol Chem. 2008, 283: 22700-22708. 10.1074/jbc.M801765200.

    PubMed Central  CAS  PubMed  Google Scholar 

  116. Koukourakis MI, Giatromanolaki A, Harris AL, Sivridis E: Comparison of metabolic pathways between cancer cells and stromal cells in colorectal carcinomas: a metabolic survival role for tumor-associated stroma. Cancer Res. 2006, 66: 632-637. 10.1158/0008-5472.CAN-05-3260.

    CAS  PubMed  Google Scholar 

  117. Michelakis ED, Webster L, Mackey JR: Dichloroacetate (DCA) as a potential metabolic-targeting therapy for cancer. Br J Cancer. 2008, 99: 989-994. 10.1038/sj.bjc.6604554.

    PubMed Central  CAS  PubMed  Google Scholar 

  118. Madhok BM, Yeluri S, Perry SL, Hughes TA, Jayne DG: Dichloroacetate induces apoptosis and cell-cycle arrest in colorectal cancer cells. Br J Cancer. 2010, 102: 1746-1752. 10.1038/sj.bjc.6605701.

    PubMed Central  CAS  PubMed  Google Scholar 

  119. Bonnet S, Archer SL, Allalunis-Turner J, Haromy A, Beaulieu C, Thompson R, Lee CT, Lopaschuk GD, Puttagunta L, Bonnet S, Harry G, Hashimoto K, Porter CJ, Andrade MA, Thebaud B, Michelakis ED: A mitochondria-K + channel axis is suppressed in cancer and its normalization promotes apoptosis and inhibits cancer growth. Cancer Cell. 2007, 11: 37-51. 10.1016/j.ccr.2006.10.020.

    CAS  PubMed  Google Scholar 

  120. Xie J, Wang BS, Yu DH, Lu Q, Ma J, Qi H, Fang C, Chen HZ: Dichloroacetate shifts the metabolism from glycolysis to glucose oxidation and exhibits synergistic growth inhibition with cisplatin in HeLa cells. Int J Oncol. 2011, 38: 409-417.

    CAS  PubMed  Google Scholar 

  121. Roche TE, Hiromasa Y: Pyruvate dehydrogenase kinase regulatory mechanisms and inhibition in treating diabetes, heart ischemia, and cancer. Cell Mol Life Sci. 2007, 64: 830-849. 10.1007/s00018-007-6380-z.

    CAS  PubMed  Google Scholar 

  122. DeBerardinis RJ, Mancuso A, Daikhin E, Nissim I, Yudkoff M, Wehrli S, Thompson CB: Beyond aerobic glycolysis: transformed cells can engage in glutamine metabolism that exceeds the requirement for protein and nucleotide synthesis. Proc Natl Acad Sci USA. 2007, 104: 19345-19350. 10.1073/pnas.0709747104.

    PubMed Central  CAS  PubMed  Google Scholar 

  123. DeBerardinis RJ, Lum JJ, Hatzivassiliou G, Thompson CB: The biology of cancer: metabolic reprogramming fuels cell growth and proliferation. Cell Metab. 2008, 7: 11-20. 10.1016/j.cmet.2007.10.002.

    CAS  PubMed  Google Scholar 

  124. Mullen AR, Wheaton WW, Jin ES, Chen PH, Sullivan LB, Cheng T, Yang Y, Linehan WM, Chandel NS, DeBerardinis RJ: Reductive carboxylation supports growth in tumour cells with defective mitochondria. Nat. 2012, 481: 385-388.

    CAS  Google Scholar 

  125. Metallo CM, Gameiro PA, Bell EL, Mattaini KR, Yang J, Hiller K, Jewell CM, Johnson ZR, Irvine DJ, Guarente L, Kelleher JK, Vander Heiden MG, Iliopoulos O, Stephanopoulos G: Reductive glutamine metabolism by IDH1 mediates lipogenesis under hypoxia. Nat. 2012, 481: 380-384.

    CAS  Google Scholar 

  126. Wise DR, Ward PS, Shay JE, Cross JR, Gruber JJ, Sachdeva UM, Platt JM, DeMatteo RG, Simon MC, Thompson CB: Hypoxia promotes isocitrate dehydrogenase-dependent carboxylation of alpha-ketoglutarate to citrate to support cell growth and viability. Proc Natl Acad Sci USA. 2011, 108: 19611-19616. 10.1073/pnas.1117773108.

    PubMed Central  CAS  PubMed  Google Scholar 

  127. Smith CM, Bryla J, Williamson JR: Regulation of mitochondrial alpha-ketoglutarate metabolism by product inhibition at alpha-ketoglutarate dehydrogenase. J Biol Chem. 1974, 249: 1497-1505.

    CAS  PubMed  Google Scholar 

  128. Starkov AA: An update on the role of mitochondrial alpha-ketoglutarate dehydrogenase in oxidative stress. Mol Cell Neurosci. 2013, 55: 13-16.

    PubMed Central  CAS  PubMed  Google Scholar 

  129. Butterworth RF, Giguere JF, Besnard AM: Activities of thiamine-dependent enzymes in two experimental models of thiamine-deficiency encephalopathy. 2. alpha-Ketoglutarate dehydrogenase. Neurochem Res. 1986, 11: 567-577. 10.1007/BF00965326.

    CAS  PubMed  Google Scholar 

  130. Parker WD, Haas R, Stumpf DA, Parks J, Eguren LA, Jackson C: Brain mitochondrial metabolism in experimental thiamine deficiency. Neurol. 1984, 34: 1477-1481. 10.1212/WNL.34.11.1477.

    CAS  Google Scholar 

  131. Platell C, Kong SE, McCauley R, Hall JC: Branched-chain amino acids. J Gastroenterol Hepatol. 2000, 15: 706-717. 10.1046/j.1440-1746.2000.02205.x.

    CAS  PubMed  Google Scholar 

  132. Harper AE, Miller RH, Block KP: Branched-chain amino acid metabolism. Annu Rev Nutr. 1984, 4: 409-454. 10.1146/annurev.nu.04.070184.002205.

    CAS  PubMed  Google Scholar 

  133. Harris RA, Joshi M, Jeoung NH, Obayashi M: Overview of the molecular and biochemical basis of branched-chain amino acid catabolism. J Nutr. 2005, 135: 1527S-1530S.

    CAS  PubMed  Google Scholar 

  134. Harris RA, Joshi M, Jeoung NH: Mechanisms responsible for regulation of branched-chain amino acid catabolism. Biochem Biophys Res Commun. 2004, 313: 391-396. 10.1016/j.bbrc.2003.11.007.

    CAS  PubMed  Google Scholar 

  135. Chuang DT, Chuang JL, Wynn RM: Lessons from genetic disorders of branched-chain amino acid metabolism. J Nutr. 2006, 136: 243S-249S.

    CAS  PubMed  Google Scholar 

  136. Shimomura Y, Murakami T, Nakai N, Nagasaki M, Harris RA: Exercise promotes BCAA catabolism: effects of BCAA supplementation on skeletal muscle during exercise. J Nutr. 2004, 134: 1583S-1587S.

    CAS  PubMed  Google Scholar 

  137. Baracos VE, Mackenzie ML: Investigations of branched-chain amino acids and their metabolites in animal models of cancer. J Nutr. 2006, 136: 237S-242S.

    CAS  PubMed  Google Scholar 

  138. De Bandt JP, Cynober L: Therapeutic use of branched-chain amino acids in burn, trauma, and sepsis. J Nutr. 2006, 136: 308S-313S.

    CAS  PubMed  Google Scholar 

  139. MacDonald N, Easson AM, Mazurak VC, Dunn GP, Baracos VE: Understanding and managing cancer cachexia. J Am Coll Surg. 2003, 197: 143-161. 10.1016/S1072-7515(03)00382-X.

    PubMed  Google Scholar 

  140. Goodlad GA, Clark CM: Leucine metabolism in skeletal muscle of the tumour-bearing rat. Eur J Cancer. 1980, 16: 1153-1162.

    CAS  PubMed  Google Scholar 

  141. Argiles JM, Lopez-Soriano FJ: The oxidation of leucine in tumour-bearing rats. Biochem J. 1990, 268: 241-244.

    PubMed Central  CAS  PubMed  Google Scholar 

  142. Siddiqui RA, Williams JF: The regulation of fatty acid and branched-chain amino acid oxidation in cancer cachectic rats: a proposed role for a cytokine, eicosanoid, and hormone trilogy. Biochem Med Metab Biol. 1989, 42: 71-86. 10.1016/0885-4505(89)90043-1.

    CAS  PubMed  Google Scholar 

  143. Argiles JM, Lopez-Soriano FJ: Oxidation of branched-chain amino acids in tumor-bearing rats. Biochem Soc Trans. 1989, 17: 1044-1045.

    CAS  PubMed  Google Scholar 

  144. Paxton K, Ward LC, Wilce PA: Amino acid oxidation in the tumor-bearing rat. Cancer Biochem Biophys. 1988, 9: 343-351.

    CAS  PubMed  Google Scholar 

  145. Baracos VE: Regulation of skeletal-muscle-protein turnover in cancer-associated cachexia. Nutr. 2000, 16: 1015-1018. 10.1016/S0899-9007(00)00407-X.

    CAS  Google Scholar 

  146. Shiraki M, Shimomura Y, Miwa Y, Fukushima H, Murakami T, Tamura T, Tamura N, Moriwaki H: Activation of hepatic branched-chain alpha-keto acid dehydrogenase complex by tumor necrosis factor-alpha in rats. Biochem Biophys Res Commun. 2005, 328: 973-978. 10.1016/j.bbrc.2005.01.047.

    CAS  PubMed  Google Scholar 

  147. Huang YS, Chuang DT: Down-regulation of rat mitochondrial branched-chain 2-oxoacid dehydrogenase kinase gene expression by glucocorticoids. Biochem J. 1999, 339 (Pt 3): 503-510.

    PubMed Central  CAS  PubMed  Google Scholar 

  148. Saylor PJ, Karoly ED, Smith MR: Prospective study of changes in the metabolomic profiles of men during their first three months of androgen deprivation therapy for prostate cancer. Clin Cancer Res. 2012, 18: 3677-3685. 10.1158/1078-0432.CCR-11-3209.

    PubMed Central  CAS  PubMed  Google Scholar 

  149. Lau KS, Fatania HR, Randle PJ: Regulation of the branched chain 2-oxoacid dehydrogenase kinase reaction. FEBS Lett. 1982, 144: 57-62. 10.1016/0014-5793(82)80568-1.

    CAS  PubMed  Google Scholar 

  150. Danner DJ, Wheeler FB, Lemmon SK, Elsas LJ: In vivo and in vitro response of human branched chain alpha-ketoacid dehydrogenase to thiamine and thiamine pyrophosphate. Pediatr Res. 1978, 12: 235-238. 10.1203/00006450-197803000-00016.

    CAS  PubMed  Google Scholar 

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Acknowledgement

Dr Jason Zastre was funded by the Georgia Research Alliance, Distinguished Cancer Scholar Award during the preparation of this manuscript.

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  1. Department of Pharmaceutical and Biomedical Sciences, College of Pharmacy, University of Georgia, R.C. Wilson Pharmacy Building, Athens, GA, 30602, USA

    Jason A Zastre, Rebecca L Sweet, Bradley S Hanberry & Star Ye

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Zastre, J.A., Sweet, R.L., Hanberry, B.S. et al. Linking vitamin B1 with cancer cell metabolism. Cancer Metab 1, 16 (2013). https://doi.org/10.1186/2049-3002-1-16

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Keywords

  • Thiamine
  • Transketolase
  • Vitamin
  • Metabolism
  • Cancer

Cancer & Metabolism

ISSN: 2049-3002