Skip to main content

Characterization of the different S6Ks isoforms interacting partners

mTOR signaling pathway has been related to several human diseases and disorders, including obesity, diabetes and many types of cancer. In the cell, mTOR signaling is responsible to induce cell growth and metabolism upon activation by signals from nutrients processing, such as insulin, ATP and amino acids. S6Ks, which phosphorylates ribosomal S6 protein, are effectors of the mTOR pathway. S6K family is composed of two genes (encoding S6K1 and S6K2) and different isoforms from alternative translation initiation (p70-S6K1, p85-S6K1, p54-S6K2, p56S6K2) and alternative splicing (p31-S6K1). The main function of S6Ks, upon activation, is to lead increased protein synthesis. Nevertheless, one of the main questions in this field is to address the specific roles of the different S6Ks isoforms. In this study, we overexpressed p70-S6K1, p85-S6K1 and p54-S6K2 in human cells and analyzed by immunoprecipitation, followed by mass spectrometry identification, the different interacting partners of those S6Ks isoforms. The different roles of S6Ks isoforms were also evaluated in an adipocyte model and in cancer cell lines. Our study provide new possible functions to the different S6Ks isoforms which may help the understanding of their roles in the mTOR signaling pathway, metabolism and cancer.

Author information

Affiliations

Authors

Corresponding author

Correspondence to Fernando Simabuco.

Rights and permissions

Open Access  This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.

The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.

To view a copy of this licence, visit https://creativecommons.org/licenses/by/4.0/.

The Creative Commons Public Domain Dedication waiver (https://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Pavan, I., Ferreira, F., de Freitas, L. et al. Characterization of the different S6Ks isoforms interacting partners. Cancer Metab 2, P68 (2014). https://doi.org/10.1186/2049-3002-2-S1-P68

Download citation

Keywords

  • Obesity
  • Cancer Cell Line
  • Human Cell
  • Alternative Splice
  • Human Disease