Volume 2 Supplement 1

Metabolism, Diet and Disease 2014: Cancer and metabolism

Open Access

A metabolic map of hematopoietic stem cells

  • Michalis Agathocleous1,
  • Zeping Hu1 and
  • Sean Morrison1
Cancer & Metabolism20142(Suppl 1):P1

https://doi.org/10.1186/2049-3002-2-S1-P1

Published: 28 May 2014

Background

A general problem in biology is whether different types of cells in the same tissue are metabolically different from each other, and whether such differences are important for cellular function. Experiments that can comprehensively measure the cellular metabolome typically require millions of cultured cells and cannot be used with small numbers of rare cells freshly isolated from tissues. In particular, hematopoietic stem cells (HSCs), the blood forming stem cells of the bone marrow, have been intensively studied for decades but their metabolic composition is largely unknown.

Results

We have developed a method to measure metabolites in small numbers of HSCs. The HSC isolation and mass spectrometry methods have been optimized for maintenance of the metabolome during purification, sensitivity and robustness. About 50 metabolites can be quantified from 10,000 HSCs, covering a wide spectrum of the cellular metabolome. Several metabolic differences exist between HSCs and other bone marrow cells, including restricted progenitors. I am investigating the role of these metabolic adaptations in HSCs.

Conclusion

The ability to profile the metabolome of rare cells isolated directly from tissues opens the possibility to metabolically compare stem cells to other purified populations of cells at different stages of differentiation, to test the metabolic consequences of physiological challenges like aging on specific populations of cells, and to test whether other rare cells, like subpopulations of cancer cells from the same tumor, are metabolically distinctive.

Authors’ Affiliations

(1)
Children’s Research Institute, UT Southwestern Medical Center

Copyright

© Agathocleous et al; licensee BioMed Central Ltd. 2014

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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