Figure 3
From: Respiratory complex I is essential to induce a Warburg profile in mitochondria-defective tumor cells
Transcriptional profile of OST-93 and OST-93ND1xenografts. (A) Heatmap displaying expression levels of the 521 differentially expressed (DE) genes in the samples analyzed. Dark red = upregulated genes; cyan = downregulated genes. (B) The transcriptional profile of the Warburg phenotype. Heatmap showing gene expression profile of 21 DE genes from the hypoxia inducible factor-1α (HIF-1α) activation pathway. Genes are ordered by decreasing log2 fold change. Glucose transporters and glycolytic genes are marked in red. (C) Glucose uptake and glycolytic reactions. Genes overexpressed in OST-93ND1 xenografts are labeled in red. Red circles represent glucose molecules. (D) Quantitative real-time PCR validation performed on biological replicates of OST-93 (n = 2) and OST-93ND1 (n = 7) for 9/21 HIF-1α-responsive genes found DE in RNA-Seq (*P <0.05).