Fig. 2

H3K27M cells have highly active purine synthesis. A Schematic of purine synthetic metabolic pathways showing where the purine de novo and salvage pathways converge to form mature purines. Schematic was created using BioRender.com. B-E Total abundance of ¹³C-labeled (B) F16BP, (C) R5P, (D) GMP, and (E) AMP in H3K27M cells when normalized to H3K27M-KO cells. F Total abundance of ¹⁵N-Gln-labeled GMP (left) and AMP (right) in H3K27M-isogenic cells. H Total abundance of deuterium-labeled GMP (left) and AMP (right) in H3K27M-isogenic cells. H Ratio of ¹⁵N-Gln:2D-Hpx labeled relative abundance of GMP (left) and AMP (right) in untreated DIPGXIII H3K27M-isogenic cell lines. Statistical analyses were performed using two-tailed t-tests in GraphPad Prism 10.0