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Fig. 5 | Cancer & Metabolism

Fig. 5

From: A HIF-1α inhibitor combined with palmitic acid and L-carnitine treatment can prevent the fat metabolic reprogramming under hypoxia and induce apoptosis in hepatocellular carcinoma cells

Fig. 5

Regulation of fatty acid metabolism-related genes by HIF-1α under hypoxia. A qRT-PCR analysis of mRNA expression levels of genes encoding fatty acid β-oxidation (FAO)-related enzymes ACSL1, CPT1, MCAD, and LCAD, as well as fatty acid transporter CD36, in HIF-1α knockdown (KD) and scramble control (SC) HepG2 and Hep3B cells. B Western blot analysis of ACSL1, CPT1, MCAD, LCAD, and CD36 protein expression in KD and SC cells. C Quantification of Western blot analysis of ACSL1, CPT1, MCAD, LCAD, and CD36 protein expression. D qRT-PCR analysis of mRNA levels of triglyceride (TG) synthesis-related genes APGAT and DGAT in KD and SC cells. E Western blot analysis of APGAT and DGAT protein expression in KD and SC cells. F Quantification of Western blot analysis of APGAT, DGAT protein expression. Data are expressed as the mean ± standard error of the mean (SEM) and are representative of three independent experiments (A, D). N.S.: not significant, *P < 0.05, **P < 0.01 versus control

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Cancer & Metabolism

ISSN: 2049-3002