Fig. 5
From: Aspirin reprogrammes colorectal cancer cell metabolism and sensitises to glutaminase inhibition
Aspirin and CB-389 in combination reduce colon crypt proliferation in vivo. a Schematic summarising the effects of aspirin treatment on metabolic reprogramming of CRC cells. Created with BioRender.com. b Schematic showing the timeline of treatment, induction with tamoxifen (2 mg) and sampling for in vivo aspirin (2.6mg/ml in drinking water) and CB-839 (200mg/kg) combination experiments using the VillinCreER Apcfl/fl mouse model. c, d Quantification of BrdU and representative images of BrdU staining in the small intestine of VillinCreER Apc.fl/fl mice treated with vehicle, CB-839 (200mg/kg) and/or aspirin (2.6mg/ml in drinking water). Scale bar 100μm. Error bars show SEM (n = 4 mice per experimental arm). Each dot represents the average number of Brd-positive cells per half crypt for each mouse. Asterisks refer to p values obtained from one-tailed Mann–Whitney tests (*p < 0.05; **p < 0.01)