Fig. 7
From: Cholesterol reprograms glucose and lipid metabolism to promote proliferation in colon cancer cells
LDLc and HDLc regulate glycolytic function, mitochondrial respiration, and ATP production in colon cancer cells. HCT-116/ HCT-15 cells were pre-treated with 50 μg/ml LDLc/HDLc for 12 h in 1% FBS-containing media followed by the analysis of various metabolic parameters in the presence of 50 μg/ml LDLc/HDLc through XeF24 seahorse analyzer. A,B Glycolytic stress (ECAR) functional analysis in HCT-15 and HCT-116 respectively. Experiments were performed twice in triplicate (except for the HCT-116 control condition, done in duplicate). C Graph showing mitochondrial stress (OCR) functional analysis in HCT-116 cells. Experiments were performed twice in triplicate. D Seahorse XeF real-time ATP rate analysis of HCT-116 cells upon the vehicle or LDLc or HDLc treatment. The experiment was done in triplicate