Fig. 6
From: Cholesterol reprograms glucose and lipid metabolism to promote proliferation in colon cancer cells
LDLc and HDLc interfere with enzymes and molecules involved in glucose metabolism, i.e., lactate production and transporter in colon cancer cells. Cells were treated with different concentrations of LDLc or HDLc or vehicle and enzyme activity assay, immunofluorescence, and western blotting were performed. A,B Lactate dehydrogenase enzyme activities upon A HDLc and B LDLc treatment in HCT-116 cells. C Confocal images of HCT-116 cells probed with MCT-4. Experiments were done in triplicate. D Immunoblot analysis of MCT-1, MCT-2, MCT-4, and LDHA in HCT-116 whole cell lysate. β-tubulin, Actin, and GAPDH were used as loading controls. The experiment was performed twice