Fig. 3
NIS can non-invasively image oxidative phosphorylation inhibition in vivo using PET. a Bland-Altman analysis of tumour [18F]TFB-PET imaging quantification performed in a cohort of mice (n = 4) at baseline and 24 h later, immediately after oral administration of vehicle. Bias and 95% lines of agreement (LoA) are indicated. b Example [18F]TFB-PET MRI imaging performed 24 h before (‘pre’) and immediately after (‘post’) the administration of the vehicle (left) or IACS-10759 (right). Static PET images were acquired between 70 and 90 min from the injection of [18F]TFB and maximum standardised uptake values (SUVmax) from the region of interest (ROI) placed over the tumour were calculated using VivoQuant image analysis suite (indicated by dashed yellow line). c Summary of experiments performed in cohorts (each n = 4) treated with a single dose of vehicle (left) or IACS-10759 (right), as described in a. Two-way, repeated measures ANOVA with Å Ãdák's multiple comparisons analysis was performed to test statistical significance of the results. P value classifications are summarized as follows: *, P ∈ (0.01–0.05 〉; **, P ∈ (0.001–0.01 〉. Each data point represents SUVmax calculated for a single tumour/animal and time-point. Error bars represent one standard deviation