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Table 1 Specific primer sequences

From: Restoring gluconeogenesis by TEF inhibited proliferation and promoted apoptosis and immune surveillance in kidney renal clear cell carcinoma

Gene

Forward primer

Reverse primer

 

ACTB

CATGTACGTTGCTATCCAGGC

CTCCTTAATGTCACGCACGAT

 

ALDOB

TGTCTGGTGGCATGAGTGAAG

GGCCCGTCCATAAGAGAAACTT

 

FBP1

GAACCGGAGAAAAGGGGTAAAT

GTTCCAACGGACACAAGGCA

 

PFKM

GGTGCCCGTGTCTTCTTTGT

AAGCATCATCGAAACGCTCTC

 

PCK1

AAAACGGCCTGAACCTCTCG

ACACAGCTCAGCGTTATTCTC

 

G6PC1

CACTTCCGTGCCCCTGATAA

AGTATACACCTGCTGTGCCC

 

TEF

GCGAGACGCGCCTTGATAA

ATCGTAGGGGATGGTCTTGTC

 

PCK2

GGCTGAGAATACTGCCACACT

ACCGTCTTGCTCTCTACTCGT

 
 

Forward primer

Universal reverse primer

Stem-loop

MIR514B

GTTTTCTCAAGAGGGAGGC

GTGCAGGGTCCGAGGT

GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAACATGATT

MIR4477B

GTTGGGATTAAGGACATTTGTG

GTGCAGGGTCCGAGGT

GTTGGCTCTGGTGCAGGGTCCGAGGTATTCGCACCAGAGCCAACATCAAT

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Cancer & Metabolism

ISSN: 2049-3002