Fig. 2

CCHE1 was essential for melanoma cell growth. A Melanoma cells were transfected with siRNA-control or siRNA-CCHE1, and CCHE1 knockdown by siRNA was confirmed by qPCR. B, C CCK-8 assay was performed to compare the proliferation of melanoma cells expressing siRNA-control or siRNA-CCHE1. Reduced proliferation of melanoma cells with CCHE1 depletion detected by CCK-8 assay. D Clone formation assay of melanoma cells transduced with siRNA-CCHE1 was significantly inhibited. E Flow cytometry analysis showed that CCHE1 knockdown triggered melanoma cell apoptosis compared with the control cells. F A375 xenograft mouse model was established with stable cells expressing shRNA-control or shRNA-CCHE1. The tumor volume was measured twice a week. CCHE1 depletion significantly delayed the tumor growth (left panel) and representative tumors were shown. The knockdown efficacy of CCHE1 was confirmed by RT-qPCR analysis at the end of the study (middle panel). Tumor weight was weighted (right panel). **p < 0.01, ***p < 0.001 vs. shRNA-control