Table 6 Glutamine, TCA cycle, malic enzyme, and GSH/fatty acid synthesis fluxes

Gln and Glu metabolism

J_10a: Gln to Glu

0.94±0.06 ^a

2.04±0.06 ^b

1.02±0.03 ^a

1.60±0.08 ^c

J_10b: Gln to Other

0.29±0.06 ^a

1.78±0.14 ^b

0.64±0.07 ^a

1.84±0.30 ^b

J_11a: αKG from GDH

0.77±0.05 ^a

1.65±0.04 ^b

0.87±0.03 ^a

0.17±0.10 ^c

J_11b: αKG from J₁₉

0±0 ^a

0±0 ^a

0±0 ^a

1.43±0.06 ^b

J₁₈: Glu to GSH

0.17±0.02 ^a

0.39±0.02 ^b

0.15±0.01 ^a

0±0 ^c

TCA cycle, ME, and FA synthesis

J₅: Pyr to Cit

1.11±0.04 ^a

4.15±0.13 ^b

0.53±0.07 ^c

0.85±0.05 ^a

J₁₇: OAA to Cit

1.11±0.04 ^a

4.15±0.13 ^b

0.53±0.07 ^c

0.85±0.05 ^a

J_12f: Cit to αKG

0.88±0.04 ^a

1.67±0.05 ^b

0.18±0.08 ^c

0.08±0.04 ^c

J_12r: αKG to Cit

0.39±0.04 ^a,c

1.21±0.06 ^b

0.50±0.02 ^a

0.32±0.03 ^c

J₁₃: Cit to FA

0.62±0.04 ^a

3.68±0.15 ^b

0.85±0.03 ^a,c

1.09±0.02 ^c

J₁₄: αKG to Suc

1.26±0.05 ^a

2.12±0.05 ^b

0.55±0.11 ^c

1.36±0.13 ^a

J₁₅: Suc to Mal

1.26±0.05 ^a

2.12±0.05 ^b

0.55±0.11 ^c

1.36±0.13 ^a

J₁₆: Mal to OAA

0.88±0.04 ^a

1.67±0.05 ^b

0.18±0.08 ^c

0.08±0.04 ^c

J₇: Mal to Pyr

0.38±0.02 ^a

0.45±0.04 ^a

0.37±0.04 ^a

1.29±0.09 ^b

ATP consumers

21.7±1.0 ^a

65.7±3.1 ^b

36.1±0.9 ^c

34.2±2.3 ^c