Fig. 2

Arginase activity is related to intracellular l-arginine levels and the migration ability of colon cancer cells in vitro. A CT26 cells (2 × 10³) were cultured in the absence and presence of nor-NOHA (0, 125, 250, 500 μM). Living cell numbers were evaluated at 12 h and 24 h. The mean values and SDs (n = 4) are indicated. *P < 0.05 by Student’s t-test. B Apoptosis was evaluated by flow cytometry using 7AAD and Annexin V staining. Representative profiles are indicated. The percentage of apoptotic cells was calculated, and the mean values and SDs (n = 4) of the data are indicated. *P < 0.05 by Student’s t-test. C CT26 cells (1 × 10⁴) were cultured in the absence and presence of nor-NOHA (0, 125, 250, 500 μM). Migration ability was evaluated by Transwell assay at 24 h. Representative images are indicated. Bars represent 200 μM. The mean values and SDs (n = 4) of the data are indicated. *P < 0.05 by Student’s t-test. D N-cadherin and E-cadherin protein expression levels were determined by Western blotting. Representative images are indicated. The mean values and SDs (n = 3) of relative expression levels against α-tubulin are indicated. *P < 0.05 by Student’s t-test