Fig. 7
PDK1 and PDK2 knockdown inhibited migration ability and reversed cisplatin and gemcitabine resistance in SAS spheroids cells. A SAS shLuc, shPDK1#261, and shPDK2#315 cells (1×104 cells/2 ml) were seeded in 6-well ultra-low attachment plates in sphere medium with or without 10 ng/ml TGF-β1 sphere medium and cultured for 12 days. Migration activities of the spheroid cells (2.5×104 cells/100 μl 0.5% FBS DMEM) were determined after 30 h. Five fields were counted per filter in each group. B–D Parental (P) or spheroid (1S) cells of SAS shLuc, shPDK1#261, and shPDK2#315 cells (2×103 cells/100 μl) were seeded in 96-well white plates with different chemo drugs. Celltiter-Glo Luminescent Cell Viability Assay was accessed after 48-h incubation. shLuc (1S) compared with shLuc (P): *p < 0.05; **p < 0.01; ***p < 0.005; ****p < 0.0001. shPDK (1S) compared with shLuc (1S): #p < 0.05; ##p < 0.01