Fig. 5
PDK1 and PDK2 knockdown decreased TGF-β1-promoted migration and invasion ability of HNC cells. A Transwell migration activities of SAS shLuc, shPDK1#261, and shPDK2#315 cells (2.5×104/100 μl serum-free DMEM) and FaDu shLuc, shPDK1#263, and shPDK2#315 cells (3×104/100 μl serum-free DMEM) were determined after 30 h. B Transwell invasion activities of SAS shLuc, shPDK1#261, and shPDK2#315 cells (3×104 cells/500 μl serum-free DMEM) and FaDu shLuc, shPDK1#263, and shPDK2#315 cells (4×104 cells/500 μl serum-free DMEM) were determined after 48 h. C SAS shLuc, shPDK1#261, and shPDK2#315 cells (4×105/3 ml) were pretreated with 5 ng/ml TGF-β1 medium for 48 h. Migration activities of these cells were determined as described in A. D SAS and FaDu cells (4×105/3 ml) were pretreated with TGF-β1 medium for 48 h. Invasion activities of these cells were determined as described in B. *p < 0.05; **p < 0.01; ***p < 0.005; ****p < 0.0001