Fig. 4

PDK2 knockdown suppressed cell proliferation and clonogenic ability of HNC cells. A SAS shLuc, shPDK1, and shPDK2 cells (3000 cells/100 μl) were seeded in a 96-well plate. Cell viability was examined by MTS assay on a different day. B SAS shLuc, shPDK1, and shPDK2 cells (50 cells/3 ml) were seeded in a 6-well plate for 10 days. Clonogenic ability was determined by colony number. C SAS shLuc, shPDK1#261, and shPDK2#315 cells (3000 cells/100 μl) were seeded in a 96-well plate with or without 5 ng/ml TGF-β1. Cell viability was examined by MTS assay. D SAS shLuc, shPDK1#261, and shPDK2#315 cells (50 cells/3 ml) were seeded in a 6-well plate with or without TGF-β1 for 10 days, and the colony number was calculated. *p < 0.05; **p < 0.01