Fig. 6
Tadalafil enhanced the efficacy of 5-FU in CRC cells. A and B % of cell proliferation of SW480 (A) and HCT116 (B) treated with 5 mg/mL 5-FU and 0, 20, 40, and 60 μM tadalafil for 12 h was evaluated by MTT assay. Mean ± SEM of three independent experiments is shown. C–G Colorectal cancer xenograft model in nude mice. Nude mice were divided into four groups: control group, 5-FU group, tadalafil group, and combination group. n = 6. C Tumor pictures of nude mice. D Tumor volume growth curve; tumor volume (mm.3) = short diameter2 × long diameter/2. E Fold change relative to control of glycolytic enzymes in tumor samples. Mean ± SEM of three independent experiments is shown. F Western blotting analysis to assess LDHA levels in tumor samples. β-actin was used as loading control, and one representative experiment out of three is shown. G IHC staining of LDHA in tumor samples. Scale bar (red line) = 50 μm. *P < 0.05; **P < 0.01; ***P < 0.001