Fig. 2
Irinotecan activates both respiration and glycolysis in HCT116 cells. p53 wild-type (HCTwt) and isogenic p53 null (HCTΔp53) HCT116 cells were exposed to 10 µM irinotecan (Iri) for 24 h. A Oxygen consumption (OCR) and B extracellular acidification rates (ECAR) were assessed with a Cell Mito Stress Test using a Seahorse XFe24 Analyzer. 2 µM oligomycin, 2 µM FCCP, and 2 µM antimycin A were injected consecutively during the measurement. C Bioenergetic parameters, i.e., ATP production and respiratory spare capacity, were calculated from OCR/ECAR curves shown in A and B. D OCR and E ECAR were assessed with a Glycolysis Stress Test using a Seahorse XFe24 Analyzer. 10 mM d-glucose, 2 µM oligomycin, and 50 mM 2-DG were injected consecutively during the measurement. F Bioenergetic parameters, i.e., glycolysis, respiration difference (before and after glucose injection) and glycolytic reserve, were calculated from OCR/ECAR curves shown in D and E. All figures show the average of 3 individual experiments ± SEM. Statistics for this figure: *p < 0.05; **p < 0.01; ***p < 0.001