Fig. 2

Effects of reduced hyperinsulinemia on pancreatic cancer initiation. A–B Representative whole section (top) and high-magnification (bottom) images of PK-Ins1^-/-;Ins2^+/+ and PK-Ins1^-/-;Ins2^+/- male and female pancreata stained with hematoxylin and eosin. Scale bars: 2 mm (top) and 0.1 mm (bottom). C–D Quantification of percent of total pancreatic area occupied by PanINs and tumor in male (blue colors) and female (orange colors) PK-Ins1^-/-;Ins2^+/+ and PK-Ins1^-/-;Ins2^+/- mice (n = 14–22) (dark blue and dark orange dots denote mice that developed PDAC). Correlations of composite PanINs plus tumor area with fasting insulin levels E–F, with fasting glucose levels G–H, or with body weight I–J in male and female PK-Ins1^-/-;Ins2^+/+ and PK-Ins1^-/-;Ins2^+/- mice (n = 10-22)