Fig. 6

Tumor xenograft growth studies. A Schematic showing subcutaneous tumor growth analysis in mice fed with regular (Reg) or ketogenic high-fat (Hi-Fat) diet. Mice were treated daily once with vehicle, 5 mg/kg IACS, 1 mg/kg STN or their combinations for the number of days shown in (C–F). Tumor harvests for molecular studies were performed on the fourth (A375R1) or sixth (UCSD354L) day of treatments. B Heatmap showing unsupervised clustering analysis of significantly (p < 0.005) altered proteins in A375R1 tumors harvested from mice fed with regular (Reg) or high-fat keto diet and analyzed by RPPA. Heatmap represents Pearson correlation of significantly (p < 0.005) different protein levels in tumors from mice fed with high-fat keto (Hi-fat keto) versus regular (Reg) diet. Intensity ranges of lowest (blue) and highest (red) protein levels are indicated at the bottom of the heatmap. C and D A375R1 (C) and UCSD354L (D) tumor growth inhibition by IACS and STN treatments in mice fed with regular (Reg) diet. E and F A375R1 (E) and UCSD354L (F) tumor growth inhibition by IACS and STN treatments in mice fed with high-fat keto diet. Tumor volumes were recorded on the days shown on the x-axis, and tumor growth was represented as line graphs. Tumor growth data is from eight mice/group, error bars, standard error of mean (SEM). G and H Supervised clustering analysis of treatment-induced alterations of RPPA-analyzed proteins in tumors from mice fed with Reg (G) and Hi-Fat keto (H) diets. Heatmaps show Pearson correlation of significantly (p < 0.005) altered protein levels presented as ratios of inhibitor-treatments versus vehicle-treatments. Intensity ranges of lowest (blue) and highest (red) protein levels are indicated at the bottom of the heatmaps