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Fig. 5 | Cancer & Metabolism

Fig. 5

From: Novel humanized monoclonal antibodies for targeting hypoxic human tumors via two distinct extracellular domains of carbonic anhydrase IX

Fig. 5

A The effect of selected humanized antibodies (CA9hu-1(HC4LC4) and CA9hu-2(HC4LC5)) on the viability of analyzed cells ± expressing CA IX (C33-a CA IX versus C33-a neo) in the presence of complement determined via Cell Titer Blue Viability Assay. Cancer cells incubated in the absence of humanized antibodies are marked as “no Ab.” Data in the graph represent mean ± standard deviation values. Statistical significance was assessed using Student’s t-test (*P < 0.05, **P < 0.01, ***P < 0.001), n = 2. B Graphical representation of ADCP data. CA9hu-1 and CA9hu-2 antibody variant-dependent cell-mediated phagocytosis. Comparison of CA IX-negative versus CA IX-positive target cell line samples. Statistical significance was assessed using Student’s t-test (*P < 0.05, **P < 0.01, ***P < 0.001), n = 3

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Cancer & Metabolism

ISSN: 2049-3002