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Fig. 5 | Cancer & Metabolism

Fig. 5

From: Prostate cancer cell proliferation is influenced by LDL-cholesterol availability and cholesteryl ester turnover

Fig. 5

Inhibition of neutral cholesteryl ester hydrolysis activity slows C4-2B and PC3 cell growth in CS-FCS-containing media. a C4-2B cholesteryl ester levels following 24 h culturing in media supplemented with 10% FCS, 10% CS-FCS, and 10% CS-FCS plus 1 μM of the nCEH1 inhibitor JW480 (CS-FCS+nCEH1i). b PC3 cholesteryl ester levels following 24 h culturing in media supplemented with 10% FCS, 10% CS-FCS, 10% CS-FCS plus 1 μM of the nCEH1 inhibitor JW480, and 1 μM of the HSL inhibitor 76-0079 (CS-FCS+nCEH1i+HSLi). c C4-2B cell proliferation cultured in media containing 10% FCS, 10% CS-FCS, or CS-FCS+nCEH1i+HSLi by IncuCyte and MTT. d PC3 cell proliferation cultured in media containing 10% FCS, 10% CS-FCS, or CS-FCS+nCEH1i+HSLi by IncuCyte and MTT. Data are presented as mean ± SEM of at least three independent experiments performed in triplicate. * P ≤ 0.05 vs. FCS; # P ≤ 0.05 vs. CS-FCS by one-way ANOVA (a and b) or two-way ANOVA (c and d) followed by Tukey’s multiple comparisons test

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Cancer & Metabolism

ISSN: 2049-3002