Fig. 1
BAM15 mediated mitochondrial uncoupling reduces cell viability, proliferation, and migration in MDA-MB-231 and EO771 cells. Change in cellular proliferation over 4.5 days of continuous exposure to varying concentrations of BAM15 in A MDA-MB-231 (N=10 for Veh, 2.5, 5, and 10 1 μM BAM15, N=11 for 0.5 and 1 μM BAM15, N=12 for 15 and 20 μM BAM15) and B EO771 cells (N=10 per condition). Wound healing rate after continuous exposure to varying concentrations of BAM15 in C MDA-MB-231 (N=3 per condition) and D EO771 cells (N=6 per condition). Caspase 3/7 activity after 16-h exposure to varying concentrations of BAM15 in E MDA-MB-231 cells and F EO771 cells (N=12 per condition). Inhibition of cell viability (IC50,) after 24-h continuous exposure to varying concentrations of BAM15, doxorubicin, or cyclophosphamide in G MDA-MB-231 cells and H EO771 cells (N=12 per condition). Panels A, B, C, D, E, and F are shown as the mean ± SEM and were assessed by one-way ANOVA with Tukey’s multiple comparisons. **p<0.01, ***p<0.001,****p<0.0001. Abbreviations: IC50, half maximal inhibitory concentration