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Fig. 5 | Cancer & Metabolism

Fig. 5

From: Detection of glucose-derived d- and l-lactate in cancer cells by the use of a chiral NMR shift reagent

Fig. 5

CEST spectra of the Yb3 complex (10 mM) containing 3353T3 cancer cell-cultured media which were obtained via incubation cells with 5 mM methylglyoxal (MG) in DMEM basal media (4 h at 37°C, pH ~ 7.0). The CEST peak at 165 ppm reflects d-lactate while the peak at 154 ppm reflects l-lactate in each cell line. Glo1 re-expressed cells, sgGlo1 pLHCX (black), Glo1 deleted cells, sgGlo1 (blue), and parental cells (red)

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Cancer & Metabolism

ISSN: 2049-3002