Fig. 3
From: High-resolution spatiotemporal pHe and pO2 imaging in head and neck and oesophageal carcinoma cells
Spatial resolution of cell types based on O2 consumption measured using STO2p-Q. a 7.5 × 104 cells (FaDu, Kyse30, Cal33, Cal27, HNEpC, HFF) were seeded into 4-well silicon inserts, as shown on the left. Heat maps represent pO2 after 2 and 12 min, measured using STO2p-Q. Noise filter was applied with a smoothing factor of 2 (2-min timepoint). b Different cell numbers of FaDu, Kyse30, Cal33, and Cal27 were seeded into 4-well silicon μ-inserts (k, thousand; e.g. 8.8k = 8800 cells). Heat maps represent pO2 after 10 and 60 min. Noise filter was applied with smoothing factor of 2. Scale bar, 5 mm. HFF and Kyse30 (c) or FaDu (d) cells expressing mCherry were seeded into a 6-well plate to create different 2D-landscapes. pO2 heat maps and microscopic images were overlaid, and the borders of cell layers outlined in white (Kyse30/FaDu) and blue (HFF) based on phase contrast images (BF, bight field) and mCherry signal. Heat maps represent pO2 after 1 and 3 min (c) or after 3 and 8 min (d). Scale bars, 5 mm. HFF cells were seeded on Kyse30 (e) or FaDu (f) colonies expressing mCherry growing in 6-well plates. pO2 heat maps and microscopic images were overlaid, and the Kyse30/FaDu colonies outlined in black based on phase contrast images (BF, bight field) and mCherry signal. Heat maps represent pO2 after 2 and 10 min. Scale bars, 2.5 mm. For all measurements, representative images are shown