Fig. 2
From: High-resolution spatiotemporal pHe and pO2 imaging in head and neck and oesophageal carcinoma cells
OCR and ECAR measurements in HNSCC and normal cells. a OCR measured using STO2p-Q of FaDu, Kyse30, Cal33, Cal27, HNEpC, and HFF cells. OCR was normalised by dividing the OCR (decrease of pO2 (%)/time (s)) by the cell number and multiplying with a factor of 107 (normalised OCR). FaDu, n = 6; Kyse30, n = 5; Cal33, n = 6; Cal27, n = 6; HNEpC, n = 6; and HFF, n = 4, from independent experiments. b OCR measured using extracellular flux analysis (Seahorse). Shown are basal OCR. n ≥ 12, from two independent experiments. c ECAR measured using STO2p-Q of FaDu, Kyse30, Cal33, Cal27, HNEpC, and HFF cells. ECAR was normalised by dividing the ECAR (decrease of mpH/time (min)) by the cell number and multiplying with a factor of 104 (normalised ECAR). n ≥ 5, from independent experiments. d ECAR measured using extracellular flux analysis (Seahorse). Shown is the ECAR after injection of 5 mM glucose. n = 12, from two independent experiments. Data was plotted as dot plots; horizontal black line indicates the mean. Statistical analysis was performed to compare different cell lines (one-way ANOVA with post hoc Tukey HSD). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001