Table 1 The primer sequences and the concentration used in qRT–PCR
From: Glutaminolysis dynamics during astrocytoma progression correlates with tumor aggressiveness
Genes | Forward primer (5′–3′) | Reverse Primer (5′–3′) | Concentration (nM) |
|---|---|---|---|
GLS | CAGGGCAGTTTGCTTTCCAT | GAGACCAGCACATCATACCCAT | 200 |
GLSiso1 | GCAGAGGGTCATGTTGAAGTTGT | GGTGTCCAAAGTGCAGTGCTT | 200 |
GLSiso2 | ATCCTCGAAGAGAAGGTGGTGA | GCAAGTTCTTGTTGGAGACTTTCA | 400 |
GLS2 | ATCCTCGAAGAGAAGGTGGTGA | ATGGCTGACAAGGCAAACCT | 200 |
GLUD1 | TGGCATACACAATGGAGCGT | TCTCAATGGCATTAACATAGGCA | 400 |
GOT1 | CTGTGCCCAGTCCTTCTCCA | GATGCTCTCAGGTTCTTTTCCAA | 400 |
GOT2 | CTTGAGGTTGGAGACCAGTTGAGT | GATTGCTGCTGCCATTCTGA | 400 |
GPT2 | GGCTTTGGGCAGAGGGAA | TCACGCGTACTTCTCCAGGAA | 200 |
HPRT | TGAGGATTTGGAAAGGGTGT | GAGCACACAGAGGGCTACAA | 200 |
GUSβ | AAATACGTGGTTGGAGAGCTCATT | CCGAGTGAAGATCCCCTTTTTA | 400 |
TBP | AGGATAAGAGAGCCACGAACCA | CTTGCTGCCAGTCTGGACTGT | 200 |