Table 1 Co-expressed proteins with DHODH

UMPS

Uridine 5′ monophosphate synthase; in the C-terminal section; belongs to the OMP decarboxylase family (480 aa)

CAD

CAD protein. This protein is a “fusion” protein encoding four enzymatic activities of the pyrimidine pathway (GATase, CPSase, ATCase, and DHOase); in the central section; belongs to the metallo-dependent hydrolases superfamily. DHOase family. CAD subfamily (2225 aa)

DHFR

Dihydrofolate reductase; key enzyme in folate metabolism. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway. Catalyzes an essential reaction for de novo glycine and purine synthesis and for DNA precursor synthesis. Binds its own mRNA and that of DHFR2 (187 aa)

GMPS

GMP synthase [glutamine-hydrolyzing]; Involved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division; Glutamine amidotransferase like class 1 domain containing (693 aa)

MRTO4

mRNA turnover protein 4 homolog; component of the ribosome assembly machinery. Nuclear paralog of the ribosomal protein P0, it binds pre-60S subunits at an early stage of assembly in the nucleolus and is replaced by P0 in cytoplasmic pre-60S subunits and mature 80S ribosomes (239 aa)

CYC1

Cytochrome c1, heme protein, mitochondrial. This is the heme-containing component of the cytochrome b-c1 complex, which accepts electrons from Rieske protein and transfers electrons to cytochrome c in the mitochondrial respiratory chain; Apoptosome (325 aa)

GART

Trifunctional purine biosynthetic protein adenosine-3; phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase; in the central section; belongs to the AIR synthase family (1010 aa)

GRWD1

Glutamate-rich WD repeat-containing protein 1; histone-binding protein that regulates chromatin dynamics and minichromosome maintenance (MCM) loading at replication origins, possibly by promoting chromatin openness; WD repeat domain containing (446 aa)

PWP1

Periodic tryptophan protein 1 homolog; May play an important role in cell growth and/or transcription; Belongs to the WD repeat PWP1 family (501 aa)

NDUFS8

NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial; core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). May donate electrons to ubiquinone; NADH-ubiquinone oxidoreductase core subunits (210 aa)

SSBP1

Single-stranded DNA-binding protein, mitochondrial. This protein binds preferentially and cooperatively to ss-DNA. Probably involved in mitochondrial DNA replication. Associates with mitochondrial DNA (148 aa)

BRIX1

Ribosome biogenesis protein BRX1 homolog; Required for biogenesis of the 60S ribosomal subunit (353 aa)

CTPS1

CTP synthase 1. This enzyme is involved in the de novo synthesis of CTP, a precursor of DNA, RNA, and phospholipids. Catalyzes the ATP- dependent amination of UTP to CTP with either l-glutamine or ammonia as a source of nitrogen. This enzyme and its product, CTP, play a crucial role in the proliferation of activated lymphocytes and therefore in immunity; glutamine amidotransferase like class 1 domain containing (591 aa)

POLR1C

DNA-directed RNA polymerases I and III subunit RPAC1; DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleflunomidet and probably a clamp element that moves to open and close the cleflunomidet (By similarity) (346 aa)

RRS1

Ribosome biogenesis regulatory protein homolog; involved in ribosomal large subunit assembly. May regulate the localization of the 5S RNP/5S ribonucleoprotein particle to the nucleolus; belongs to the RRS1 family (365 aa)

CYCS

Cytochrome c; electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron transport chain (105 aa)

NOP2

Probable 28S rRNA (cytosine(4447)-C(5))-methyltransferase; involved in ribosomal large subunit assembly. S-adenosyl-l-methionine-dependent methyltransferase that specifically methylates the C(5) position of cytosine 4447 in 28S rRNA (Probable). May play a role in the regulation of the cell cycle and the increased nucleolar activity that is associated with the cell proliferation (Probable); belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family (845 aa)

CMPK1

UMP-CMP kinase; catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors. Belongs to the adenylate kinase family. UMP-CMP kinase subfamily (228 aa)

SDHA

Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial; flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). Can act as a tumor suppressor; belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily (664 aa)

DDX56

Probable ATP-dependent RNA helicase DDX56; may play a role in later stages of the processing of the pre-ribosomal particle