Fig. 2

Cells can be trained to metabolize fructose for proliferation. a Schematic for the positive selection strategy to generate fructolytic cell lines. b PC3 and PC3 passage 10 (P10) cells were seeded into 96-well plate (1500 cells/well) and cultured in media containing various amounts of sugar. Cell density (% confluency) was monitored over time using live cell imaging (n = 2 per condition). c Schematic for the competition growth assay between PC3-red (parental PC3 cells transduced with RFP reporter) and fructose-trained cell lines. d 40,000 of PC3, semi-trained PC3 passage 20 (P20), and trained-PC3 cells in 10 mM fructose or 11 mM glucose over time (n = 2 per condition). e Cells from d were grown in 10 mM fructose or 11 mM glucose for 96 h. They were then fixed and stained with crystal violet solution (n = 2 per condition). f 20,000 PC3-red and 20,000 trained PC3 cells were seeded in the same well and cultured for 96 h in 10 mM fructose or 10 mM glucose-containing media. Live fluorescent imaging was performed and the proportion of PC3-red cells to total PC3 cells is shown over time (n = 2 per condition). Supplemental Video 1 and Supplemental Video 2 are of competition assays monitored with live cell imaging