Fig. 3
From: SIRT6 enhances oxidative phosphorylation in breast cancer and promotes mammary tumorigenesis in mice
SIRT6 enhances OXPHOS and energy status in MDA-MB-231 cells. a–h Expression (a) and activity (b) of pyruvate dehydrogenase (PDH), activity of mitochondrial complexes (c–e), oxygen consumption (f), activity of Fo-F1 ATP synthase (g), and energy status, expressed as ATP/AMP ratio (h), were measured in MDA-MB-231 cells transduced with human WT or catalytically inactive (H133Y) SIRT6, or with a control vector (VECTOR). Data are presented as mean ± SD of three different experiments. *P < 0.05, **P < 0.01, ***P < 0.001; ns, not statistically significant. i–p Expression (i) and activity (j) of PDH, activity of mitochondrial complexes (k–m), oxygen consumption (n), activity of Fo-F1 ATP synthase (o), and energy status, expressed as ATP/AMP ratio (p) were measured in MDA-MB-231 cells transduced with a short hairpin RNA targeting SIRT6 or with a control vector (VECTOR). Data are presented as mean ± SD of three different experiments. **P < 0.01, ***P < 0.001. q 2 × 106 MDA-MB-231 BC cells transduced with either a SIRT6-shRNA or a control vector (VECTOR) were injected subcutaneously in both flanks of BALB/c athymic nude mice. Mice were sacrificed, and tumors were excised 50 days after cell inoculation. Proteins were extracted, and phosphorylated AMPK (Thr183, Thr172), total AMPK, and vinculin were detected by Western blot. One representative experiment out of two is presented