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Fig. 3 | Cancer & Metabolism

Fig. 3

From: Intracellular arginine-dependent translation sensor reveals the dynamics of arginine starvation response and resistance in ASS1-negative cells

Fig. 3

Validating degradation of sensor. a Degradation of GFP ArgSen and deletion variants in SKLMS1 in the presence of a translation inhibitor (CHX). Error bars represent standard deviation. b Best approximations of half-lives with 95% confidence intervals calculated from data in a. ND = not determined. c Representative images of SKLMS1 GFP ArgSen fluorescence during treatment with ADI-PEG20, CHX, or a proteasome inhibitor (BTZ). d GFP ArgSen fluorescence in SKLMS1 cells with ADI-PEG20, adding CHX at various time points. Error bars represent standard deviation. e GFP ArgSen fluorescence in SKLMS1 cells with ADI-PEG20, adding BTZ at various time points. Error bars represent standard deviation. f Immunoblots of GFP ArgSen protein in SKLMS1 over ADI-PEG20 time course. g Quantification of f, along with the same data normalized to corresponding mRNA levels from 2 days. Error bars represent standard error of the mean

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Cancer & Metabolism

ISSN: 2049-3002