Fig. 2
Cancer metabolic fluxes in A549 lung cancer and HCT116 colon cancer cells. a, b Schematic representation of central carbon metabolism with net flux values estimated by 13C MFA in A549 (a) and HCT116 (b) in control condition. Arrows colors (red color = up flux, violet = down flux) and thickness represent the significantly different fluxes (based on 95% confidence intervals). c Mitochondrial respiration reflected by OCR levels in A549 ( 
) and HCT116 ( 
) cancer cells under basal conditions or following the addition of oligomycin (1 μM), the uncoupled FCCP (1 μM), or the electron transport inhibitor Rotenone (0.5 μM) (n = 5). d, e Mitochondrial ATP (d) and intracellular ROS levels (e) were measured by Seahorse assay and DCFDA staining, respectively, in A549 ( 
) and HCT116 ( 
) maintained in standard growth medium for 48 h. Error bars indicate SD (n = 3)