Fig. 4

Myc regulates G6PD levels in resistant cells. a Immunoblot depicting c-myc levels in the resistant cells. Effect of siRNA-mediated downregulation of c-myc on G6PD levels was assessed using immunoblot analysis (n = 3). b Effect of c-myc inhibition, c-myci (10058F4, c-myc inhibitor), was analyzed on G6PD levels (n = 3). c Effect of acute c-myci treatment on extracellular acidification rate of MiaPaCa/Erlo cells was analyzed using Seahorse metabolic analyzer (n = 2). d Levels of ID1 were compared in drug-sensitive and drug-resistant cells using immunoblot analysis (n = 3). e Effect of ID1 knockdown was assessed on levels of c-myc and G6PD in MiaPaCa/Erlo cells using immunoblot analysis (n = 3)