Fig. 4

Inhibition of mTORC1 activity by MEDICA. a AU565 cells were treated for 24 h with 200 μM MEDICA, followed by adding 50 ng/ml EGF for the last 15 min as indicated. Representative blots. b AU565 cells were treated for 24 h with 200uM MEDICA. Representative blots. c AU565 cells were treated with MEDICA as indicated. Representative blots. d AU565 cells were treated for 24 h with 5 nM rapamycin. Representative blots. e AU565 cells were treated for 24 h with MEDICA or torin as indicated. Representative blots. f BT474 and AU565 cells were treated for 24 h with MEDICA and rotenone (0.5 μM) as indicated. Representative blots