Fig. 3

Some pathways are specifically regulated at transcriptional or translational levels. Biplot showing the log2-fold TMM differences of RPFs (y-axis) and mRNA (x-axis) between MCF7 MKL1 ΔN200 and MCF7 MKL1 ΔC301. Genes with expression changes driven by transcription regulation are shown in blue, genes with increased translation efficiency in red, and genes with decreased translation efficiency in green. Color shades represent log10 p values resulting from the differential translation efficiency analysis: light shades indicate high values while strong shades indicate low values. Genes were considered differentially expressed if p value < 0.01 and abs(FC) > 2. The fold change cutoff value is indicated as a dashed line. Summary of the GO term enrichment analysis performed with the different group of genes between MCF7 MKL1 ΔN200 and MCF7 MKL1 ΔC301 control is shown. Selected GO classes with an overrepresentation are indicated. For genes with expression changes driven by transcription regulation upregulated and downregulated genes were used independently in the GO analysis. TMM, trimmed mean of M values