Skip to main content
Fig. 5 | Cancer & Metabolism

Fig. 5

From: Fumarate hydratase-deficient renal cell carcinoma cells respond to asparagine by activation of the unfolded protein response and stimulation of the hexosamine biosynthetic pathway

Fig. 5

13C enrichments from labeled Asp or Gln in UDPGlcNAc and metabolic subunits. N-Acetyl-D-Glucosamine 6-Phosphate and UDPGlcNAc were quantified for UOK262WT (WT) and UOK252FH−/− (KO) under different conditions by IC-MS as described in the “Methods” section. a Fractional enrichment in isotopologues of N-Acetyl-D-Glucosamine 6-Phosphate. b Total UDPGlcNAc in μmol/g protein. UOK262 + Q + N versus UOK262 + Q-N p = 0.0601; 262WT + Q + N versus +Q-N p = 0.0164, +Q + N versus –Q + N p = 0.296. c Fractional enrichment in isotopologues of UDPGlcNAc. Cx_Ny are isotopologues that contain x13C and y15N atoms. Upper (Panel A) and Left (Panel C): WT versus KO for 13C15N Asn alone and for 13C15N Gln alone. WT 13C15N Asn, KO 13C15N Asn, WT 13C15N Gln, KO 13C15N Gln. Lower (Panel A) and right (Panel C): WT versus KO for 13C15N Asn with 12C14N (unlabeled) Gln and for 12C14N (unlabeled) Asn + 13C15N Gln. WT 13C15N Asn plus 12C14N Gln, KO 13C15N Asn plus 12C14N Gln, WT 12C14N Asn plus 13C15N Gln, KO 12C14N Asn plus 13C15N Gln

Back to article page

Cancer & Metabolism

ISSN: 2049-3002