Fig. 3
From: Tissue of origin dictates GOT1 dependence and confers synthetic lethality to radiotherapy
Metabolic profiles of PDA and CRC following GOT1 inhibition. a Relative aspartate (Asp) and malate (Mal) pools as determined by LC/MS in iDox-shGOT1 #1 PDA and CRC, presented as GOT1 knockdown over mock (+dox/−dox). b Relative glycolysis metabolite pools, as presented in a. c Summary of changes to central carbon metabolism upon GOT1 knockdown PDA cells. Red represents increased pool sizes in PDA cells upon GOT1 knockdown, and gray represents decreased metabolite pools. d Basal extracellular acidification rate (ECAR) levels in iDox-shGOT1 #1 and control shNT PDA and CRC cells, as determined by Seahorse Metabolic Flux Analysis. e Ion currents for isotopologue distribution of Asp derived from U-13C-Glc (left) or U-13C-Gln (right) in iDox-shGOT1 #1 PA-TU-8902 PDA and DLD-1 CRC cell lines. 3PG, 3-phosphogycerate; Ac-CoA, acetyl-CoA; αKG, alpha-ketoglutarate; Ala, alanine; B(1,3)PG, 1,3-bisphosphoglycerate; B(2,3)PG, 2,3-bisphosphoglycerate; Cit, citrate; DHAP, dihydroxyacetone phosphate; F6P, fructose 6-phosphate; FBP, fructose-1,6-bisphosphate; Fum, fumarate; G6P, glucose 6-phosphate; GA3P, glyceraldehyde 3-phosphate; Iso, isocitrate; Lac, lactate; OAA, oxaloacetate; PEP, phosphoenolpyruvate; Pyr, pyruvate; Suc, succinate. Error bars represent s.d. from biological replicates (n = 3, in a, b, e; n = 5 in d). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; Student t test (unpaired, two-tailed)