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Fig. 4 | Cancer & Metabolism

Fig. 4

From: Epithelial to mesenchymal transition (EMT) is associated with attenuation of succinate dehydrogenase (SDH) in breast cancer through reduced expression of SDHC

Fig. 4

SDH enzyme inhibition causes induction of EMT. We investigated if SDH enzyme inhibition causes induction of EMT by treating MCF10A cells with the SDH inhibitor malonate (25 mM) for 3 days. a Conventional analysis of mitochondrial respiratory function by measuring oxygen consumption rate (OCR) in DMEM medium in malonate treated MCF10A cells. b The diagram shows statistical data from the experiment described in (a). c SDH-linked respiration was assessed with succinate (Succ) as the only provided substrate, following the addition of cell permeabilizing agent (PMP) and ADP. Oligomycin (Oligo) and antimycin A (AMA) was added to control mitochondrial integrity and background activity, respectively. d The diagram shows statistical data from the experiment described in (c). e mRNA and f protein expression of epithelial (E-cadherin (CDH1)) and mesenchymal (N-cadherin, (CDH2); vimentin, (VIM)) markers. g Phase contrast microscopy. Student’s t test was used for statistical analysis. Data are shown as mean ± SEM for (a)–(d) and mean ± SD for (e). *p < 0.01

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Cancer & Metabolism

ISSN: 2049-3002