Fig. 3

Only oxamate significantly inhibits LDH activity when drugs are given to purified mitochondria from HeLa cells. a Mitochondria were first purified from HeLa cells and then given oxamate (oxa) or GSK-2837808A (GSK). b Oxygen consumption rate (OCR) from purified mitochondria provided lactate as a respiratory substrate. Each condition is compared to a vehicle control having an identical preparation, but without drug treatment. c M + 2 labeling in citrate from purified mitochondria provided U-¹³C lactate as a respiratory substrate. The M + 2 isotopologue is significantly decreased due to oxamate, but not significantly different from the vehicle control due to GSK-2837808A treatment. All data shown are averages from groups of n = 3. **p < 0.01; n.s., no statistical difference