Fig. 4

R248W mutant p53 confers enhanced tumour growth and increased ROS control in serine- and glycine (SG)-depleted conditions in vivo, correlating with decreased human patient survival. a R175H or R248W tumour growth in SG or control fed mice. Data presented as iRFP level relative to day 4 baseline reading (mean ± SEM) from N = 9 R175H control, N = 8 R175H − SG, N = 8 R248W control, and N = 10 R248W − SG tumours. Data analysed using a two-way ANOVA with Tukey’s multiple comparisons test and multiplicity-adjusted p values. p value reported in figure from final measurements. b H&E staining (× 40 magnification) and p21 and MDM2 IHC staining (× 20 magnification) in tumours from -SG fed mice. Representative images from at least N = 5 tumours per group. c Quantification of p21 and MDM2 IHC staining in tumours from -SG fed mice. Each point represents the mean positive area taken from seven random images per tumour. The black bar represents the mean value per group. N = 9 tumours per group for p21 IHC and N = 5 tumours per group for MDM2 IHC. Data analysed using a paired t test for each stain. d Malondialdehyde (MDA) IHC staining (× 20 magnification) in tumours from control or − SG fed mice. Representative images shown from N = 5 tumours per group. e Quantification of MDA IHC staining in tumours from control or − SG fed mice. Each point represents the mean positive area taken from seven random images per tumour. The black bar represents the mean value per group of N = 5 tumours. Data were analysed using a two-way ANOVA with Holm-Sidak’s multiple comparisons test and multiplicity-adjusted p values. f Survival curves for patients whose cancers contained R175H (N = 156 cases) or R248 (both W and Q pooled) (N = 207 cases). Median survival (2483 days for R175H and 1481 days for R248) was compared between the groups using the Mantel-Cox Log-rank test