Fig. 1

PKM2 is expressed in normal pancreas and in PDAC tumors. a Normal mouse pancreas and mouse PDAC tumor sections from end-stage LSL-Kras^G12D/+;Trp53^flox/flox;Pdx-1-Cre (KP^−/−C) mice were stained with hematoxylin and eosin (H&E) and isoform-specific antibodies against PKM2 or PKM1 as shown. Scale bars represent 50 μm for all images at × 20 magnification (top) and 20 μm for all images at × 40 magnification (bottom). b Western blot of normal mouse pancreas and mouse PDAC tumor lysates (from KP^−/−C mice) performed using isoform-specific antibodies against PKM1 and PKM2 as well as an antibody against vinculin as a control. c Distribution of PKM2 staining intensities from a tissue microarray containing sections from 68 human pancreatic adenocarcinoma tumors