Fig. 5

HK2 deficiency impairs Th2-mediated lung inflammation in vivo. Six to eight-week old WT and T-Hk2^−/− mice were pre-sensitized to OVA twice, 14 days apart with intraperitoneal injection of OVA/alum or PBS/alum. Twenty-one days after initial pre-sensitization, mice were challenged with aerosolized OVA daily for 3 days. Mice were euthanized for analysis on the fourth day. a H&E staining of lung tissue. Scale bars show 100 um, representative images shown from n = 4 mice. b Number of indicated cells present in BAL fluid (c) ELISA for indicated cytokines in BAL fluid. b, c Multiple t tests with Holm-Sidak correction for multiple comparisons. d–h RNA expression of indicated cytokine relative to β-actin. In all panels unless otherwise noted, error bars represent mean ± SEM, biological replicates, n = 5, 8, 4, 8 mice (left to right) from three independent experiments, unless otherwise noted, one-way ANOVA with Sidak multiple comparisons correction, *p < 0.05; **p < 0.01. NS = not significant