Fig. 4

HK2 deficiency reduces T cell-mediated colon inflammation in vivo. T-Hk2^−/− mice were crossed to IL10^−/− mice to induce colitis. Mice were euthanized at approximately 13–15 weeks of age or earlier if required due to weight loss or rectal prolapse. a H&E staining and scoring of large intestine section in indicated mice. Scale bars show 50 um, representative images shown. Biological replicates, n = 2–5 mice as indicated by individual data points. b Percentage of mice which spontaneously develop rectal prolapse by 15 weeks of age. Chi-squared test. n = 4, 12, 10, 10 mice (left to right). c Fold change in weight between week 9 and euthanasia. Biological replicates, n = 4–15 mice, as indicated by individual data points. d Spleen weight. Biological replicates, n = 3–11 mice as indicated by individual data points. e-g Total number of indicated cell type isolated from mesenteric lymph nodes (e, f; n = 2–10 mice, as indicated by individual data points) and colon lamina propria (g; n = 2–7 mice, as indicated by individual data points). In all panels unless otherwise noted, error bars represent mean ± SEM, at least three independent experiments performed, one-way or two-way ANOVA with Sidak multiple comparisons correction, *p < 0.05; **p < 0.01. NS = not significant