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Table 2 Details of the primer pairs used in this study

From: Beta-hydroxybutyrate (3-OHB) can influence the energetic phenotype of breast cancer cells, but does not impact their proliferation and the response to chemotherapy or radiation

Gene Forward primer/reverse primer cDNA size (bp) PubMed access no.
β-Actin 5′-CCT TGC CAT CCT AAA AGC C-3′
5′-CAC GAA AGC AAT GCT ATC AC-3′
96 NM_001101
PPIA 5′-TGT CCA TGG CAA ATG CTG GAC CC-3′
5′-GCG CTC CAT GGC CTC CAC AA-3′
140 NM_021130.3
BDH1 5′-CGC CGG GTG AAG GCG-3′
5′-GAA TGG CCC AGT TCC TCC C-3′
92 NM_004051.4
SCOT 5′-GCC ATT GCC AGT AAG CCA AG-3′
5′-CCA GGC TTT CAC CAA AGC AA-3′
99 NM_000436.3
ACAT1 5′-CGG CAG ATG CAG CGA AGA GG-3′
5′-AGG TTC TAC AGC AGC GTC AGC A-3′
77 NM_000019.3
  1. DNA sequences and PubMed accession numbers for each gene are indicated. qPCR reactions were performed on a CFX96 real-time PCR system (Bio-Rad) operated by CFX Manager Software (version 3.0). The two-step cycler protocol was 5 min at 95 °C, 40 cycles of 15 s at 95 °C, 60 s at 60 °C, followed by 5 min at 72 °C and used for all primer pairs
  2. PPIA peptidylprolyl isomerase A, BDH 3-hydroxybutyrate dehydrogenase, SCOT succinyl-CoA transferase (3-oxoacid CoA transferase), ACAT acetyl-CoA-acetyltransferase