Fig. 5

IDH1 mutant enzyme activity does not completely define the IDH1 mutant metabolic profile. a Clustering of eight samples based on LC-MS glucose labeling as described in Fig. 2. Analysis includes the same cohorts of IDH1 mutant and wildtype cultures with the addition of IDH1 mutant overexpression in an IDH WT line and inhibition of the endogenous mutant enzyme (308 + IDH1mut and 213 + c227 respectively). b, c Quantification of glutamine and glucose consumption with IDH1 mutant overexpression and inhibition. d Contribution of glucose and glutamine to 2-HG production in endogenous and transduced IDH1 mutants. e Effects of IDH1 mutant overexpression on glutamine and TCA cycle intermediates quantified using LC-MS. p < 0.05 for all seven metabolites shown. Error bars denote ± SEM