Fig. 2

Metabolic profile of IDH1 mutant and wildtype cells. a–c Quantification of glucose and glutamine consumption as well as the ratio of glucose consumed to lactate produced. Measurements were acquired using NOVA (N = 18 IDH WT and 5 IDH1 mutant). Statistical significance was determined by Student’s t test. d Clustering of three IDH1 mutant and three IDH wildtype lines according to LC-MS glucose labeling (IDHWTs: blue, IDH1 mutants: red). A glucose labeling index for each of the 159 metabolites analyzed was calculated for all 6 cell lines followed by non-hierarchical clustering analysis. Each line was run in triplicate and all samples are shown. e Quantification of glucose labeling among nucleotide precursors by group. Relative amounts of metabolites were calculated by summing up all isotopologues of a given metabolite and normalized to the internal standard and cell number