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Fig. 1 | Cancer & Metabolism

Fig. 1

From: Elevated circulatory levels of leptin and resistin impair therapeutic efficacy of dacarbazine in melanoma under obese state

Fig. 1

Leptin and resistin impair the outcome of DTIC therapy in melanoma cells. a, b Effect of leptin (a) and resistin (b) on the outcome of DTIC in A375 cells. A375 (human melanoma) cells were plated in 96-well plates. After 24 h, cells were treated with 100 ng/ml of recombinant leptin or resistin in DMEM containing 1% FBS for 1 h. Then, cells were treated with DTIC at the indicated concentrations and incubated for 48 h. These cells were subjected to MTT assay, and IC50 of DTIC was calculated. cf Effect of ob/ob and db/db serum factors on the survival of B16F10 cells upon DTIC treatment. B16F10 cells were chronically grown in medium containing 5% serum collected from ob-WT or ob/ob mice for 15 days. These cells were then subjected to DTIC treatment at the indicated concentrations, for 48 h, and MTT assay was performed. c Survival of B16F10 cells in DMEM containing ob-WT or ob/ob serum. d Measurement of IC50 values of DTIC in DMEM containing ob-WT or ob/ob serum. e, f B16F10 cells were chronically grown in medium containing 5% serum collected from db-WT or db/db mice for 15 days. These cells were then subjected to DTIC treatment at the indicated concentrations, for 48 h, and MTT assay was performed. e Survival of B16F10 cells in DMEM containing db-WT or db/db serum. f Measurement of IC50 value of DTIC in DMEM containing db-WT or db/db serum. The data are representative of experiments performed three times in triplicate. The results are given as means ± standard error of the mean. All the experiments were performed three times. Statistical analysis was performed using two-tailed unpaired Student’s t test; *p < 0.05; Ctrl, control; Lep, leptin; Res, resistin

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