Fig. 1

Metabolomics profiling of TNBC cell lines. Hierarchical clustering of metabolic profiles of TNBC cell lines reveals molecular heterogeneity between subtypes. a Pool size measurements show common clusters of low TCA cycle and elevated amino acid metabolites of mesenchymal-like subtype cell lines MDA-MB-231 and Hs578 vs basal-like subtypes HCC70 and MDA-MB-456. b Clustering of drug responses of TNBC cell lines (average ratios of metabolite concentrations in conditions INC280/vehicle and erlotinib/vehicle are plotted for each set of biological triplicates). Drug perturbations of reduced amino acid pool sizes show similar response of reduced amino acid pool sizes upon receptor tyrosine kinase inhibitor treatment of mesenchymal-like subtype MDA-MB-231 and Hs578 cell lines. INC280/capmatinib was used to inhibit proto-oncogene MET receptor tyrosine kinase, and erlotinib was used to inhibit receptor tyrosine kinase and growth factor receptor EGFR in TNBC cell lines