Fig. 6
siRNA-mediated knockdown of ATGL and HSL in 3T3-L1 adipocytes. a Representative immunoblots of ATGL and HSL knockdown in 3T3-L1 adipocytes. Image is representative of six independent experiments. b NEFA secretion and c TAG content in 3T3-L1 adipocytes electroporated with either non-targeting (control) or LIPE (HSL) and PNPLA2 (ATGL) siRNAs (four independent experiments performed in triplicate). d Transfer of adipocyte-derived 3H-fatty acids to MCF-7 and MDA-MB-231 cells (three independent experiments performed in duplicate). e MDA-MB-231 and f MCF-7 cell proliferation co-cultured with or without ATGL/HSL knockdown 3T3-L1 adipocytes (three independent experiments performed in quadruplicate). g, h Migration of MDA-MB-231 cells co-cultured with or without ATGL/HSL knockdown 3T3-L1 adipocytes (three independent experiments performed in quadruplicate). Data are means and SEM. b–d *P < 0.05 compared to control siRNA with Student’s t test. e–g *P ≤ 0.05 vs. basal media, #P ≤ 0.05 compared to ATGL and HSL KD by two-way ANOVA repeated measures followed by Tukey’s multiple comparisons test and h *P ≤ 0.05 vs. basal media by one-way ANOVA followed by Tukey’s multiple comparisons test